Recently, I have received many inquiries about tromethamine in the backend, mainly inquiring about the pH range of tromethamine buffer and its advantages and disadvantages. In this article, AVT editor will introduce the pH range and advantages and disadvantages of injection grade tromethamine buffer to you!

Injection grade tromethamine buffer is a commonly used buffer in biochemical research, with an effective pH range in the "neutral" range, such as:

Injection grade tromethamine buffer: pH=7.5~9

Tris HCl buffer solution: pH=7.5~8.5

Tris phosphate buffer solution: pH=5.0~9.0

1. Chinese name: Aminobutriol

2. English name: Tris Base

3. Chemical name: Tris (hydroxymethyl) aminomethane

4. Product Name: Tris

5. Molecular formula: C4H11NO3

6、CAS： 77-86-1

7. Grade: Chemical reagent grade

12. Appearance: This product is a white crystal.

13. Purity:>99% (see COA for all indicators).

14. Solubility: 550 g/L water (25 ℃)

15. Molecular weight: 121.14

16. Melting point: 167-172 ℃

17. Boiling point: 219-220 ℃ 10 mm Hg

18. Specific rotation: NA

The advantages of the pharmaceutical excipient tromethamine buffer are as follows:

1. Aminobutriol base has strong alkalinity, so this buffer system can be used to prepare buffer solutions with a wide range of pH values from acidic to alkaline, with a wide range of applications;

2. Aminobutriol buffer has minimal interference with biochemical processes and does not precipitate with calcium, magnesium, and heavy metal ions;

3. Aminobutriol has high solubility in water and is inert to many enzyme reactions;

4. Aminobutriol has high buffering capacity and strong buffering ability between pH 7.5-9.0;

5. The application of tromethamine buffer plays an important role in fields such as biochemistry, molecular biology, in vitro diagnostics, cosmetics, coatings, etc.