Pharmacopoeia of the People's Republic of China2025 Edition - Determination of Ciwujia Extract Content

This product is a processed extract of Acanthopanax senticosus. [Method] Take Acanthopanax senticosus1000gCrush into coarse powder, boil twice with water, each time3After hours, combine the decoction, filter, and concentrate the filtrate into a paste50g(Water extract), obtained immediately; Or take the Thorn Five Plus1000gCrush into coarse powder and add75%Ethanol, reflux extraction12Hour, filter, recover ethanol from the filtrate until there is no alcohol odor, and concentrate into a paste40g(Alcohol extract), ready to use.

【 Appearance 】 This product is a thick, dark brown paste like substance; Fragrant aroma, slightly bitter and astringent taste.

usePositive ODS-AQ 120A 5um 4.6X250mmChromatographic column, refer to the Chinese PharmacopoeiaThe 2025 version of high-performance liquid chromatography method was used to determine the content of Ciwujia extract, detecting syringin (C17H24O9), Ciwujia glycoside E (C34H46O18), and isoquinoline (C11H10O5). The results obtained meet the experimental requirements.

Chromatographic conditions and system suitability test:

chromatographic columnPositive ODS-AQ 120A 5um 4.6X250mmm

mobile phase Using acetonitrile as the mobile phaseA， Using 0.1% phosphoric acid solution as mobile phase B, perform gradient elution as specified in the table below;