Bunsen: How much do you know about the storage method of Elisa test kits?

The storage of test kits is also crucial in ELISA experiments: Our technical editor will introduce how to store ELISA test kits.

　　ELISA kitStore immediately at 2-8 ℃ upon receipt: expiration date can be found on the label of the reagent kit; All ingredients can be stored until their expiration date at 2-8 ℃. Application: All reagents should be balanced to room temperature. Different batches of reagents cannot be used interchangeably.

1. Stop solution: 1 bottle: 12ml: 0.5MLiu acid: stored at 2-8 ℃ until expiration date.

2. Fetal globulin A tracer antibody: 1 bottle: 0.6 ml: concentrated: HRP symbol anti human fetal globulin A tracer antibody dissolved in a stable protein matrix; This reagent needs to be diluted with tracer antibody diluent for use; Store at 2-8 ℃ until expiration date.

3. Coated plate: 96 wells: coated with anti human fetal globulin A antibody; Microporous plate fixed on the plate frame: sealed in an aluminum foil sealed bag containing desiccant; Store at 2-8 ℃ until expiration date.

4. Experimental buffer: Concentrate: 1 bottle: 11ml: phosphate buffered saline containing bovine serum albumin; Advocate the use of a 1:10 ratio diluted with double distilled water (such as 11 ml concentrated+99 ml double distilled water); Store at 2-8 ℃ until expiration date.

5. Tracer antibody diluent: 1 bottle: 11ml: ready to use: Trizma hydrochloric acid dissolved in buffer solution; According to the experimental process, it is used to trace antibody dilution; Store at 2-8 ℃ until expiration date.

6. Washing solution: concentrated: 1 bottle: 20ml: 30x concentrated; The experiment requires dilution with 580ml of double distilled water: mix well; Once diluted: phosphate buffered detergent without azide preservatives will contain surfactants; Diluted detergent can be stored at room temperature until its expiration date.

7. HRP substrate solution: 1 bottle: 12ml: TMB substrate solution containing hydrogen peroxide; Store at 2-8 ℃ until expiration date.

8. Fetal globulin A standard product: 5 bottles: containing fetal globulin A: liquid bovine serum matrix without azide preservatives; The concentration of each standard product can be found on the bottle label; After 3 cycles of freezing, all standard products should be stored at -20 ℃ or lower.

Note: The above is for reference only. This reagent is for scientific research use only and is not considered as actual data. Experiments must strictly follow the instructions or consult technical teachers.

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