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Is cell cryopreservation no longer dependent on liquid nitrogen? This program cooling device provides the answer
Date: 2025-10-16Read: 18
Diancheng Biotech Dichbio
Cell cryopreservation technology, as a key link in biomedical research, directly affects experimental results and cell activity in terms of safety and controllability. Although traditional liquid nitrogen cooling is widely used, it has problems such as high pollution risk, complex operation, high cost and compliance pressure. In recent years, liquid nitrogen free program cooling technology based on Stirling engines has gradually emerged. With the advantages of precise temperature control and integrated operation, it is providing a safer and more efficient solution for cell cryopreservation.
The scientific exploration of cell freezing technology can be traced back to the 18th century. In 1776, Italian scientist Spallanzani completed a groundbreaking experiment: placing horse sperm in a glass bottle, freezing it with snow, and then warming it back, observing that the sperm regained their swimming ability. This discovery confirms that low temperatures can suspend life activities without causing irreversible death, laying the foundation for cryobiology.
With the development of technology, cooling methods have evolved fromNatural freezing, programmed cooling box, liquid nitrogen cooling deviceGradually upgrade toFree liquid nitrogen program cooling device.
The current mainstream cooling method is liquid nitrogen cooling device, but there are significant drawbacks:
Pollution risk:Liquid nitrogen medium is prone to cross contamination between instruments and samples;
Cellular riskThe more aggressive method of liquid nitrogen freezing can reduce cell viability;
Operational risk:Operators need skills and experience, which can easily cause frostbite accidents. Liquid nitrogen is highly volatile and can cause respiratory discomfort. Moreover, liquid nitrogen is a hazardous chemical and requires special management qualifications;
Cost risk:If liquid nitrogen freezing is used, additional experimental consumables such as goggles and gloves need to be purchased, and the management cost of the entire process of purchasing, transporting, and storing liquid nitrogen is high.
The Grant series CRFT liquid nitrogen free program cooling instrument from Diancheng Biology mainly uses a Stirling engine for program coolingA Stirling engine is a type of heat engine that operates by circulating compression and expansion of air or other gases (working fluids) between different temperatures, thereby converting thermal energy into mechanical work.
Compared with liquid nitrogen cooling devices, the Point CRFT Non Liquid Nitrogen Program Cooling Device has the following advantages:advantage：
Avoiding liquid nitrogen pollution and the burden of hazardous chemical management, with no release of volatile gases throughout the process;
Used in conjunction with software, precise program control of temperature changes, stability ± 0.1 ℃, uniformity ± 1.0 ℃, maximum rate 10 ℃/min;
The liquid nitrogen free program cooling device can not only cool down but also heat up, with a controllable temperature range of -100 ℃ -30 ℃, and an integrated process for freezing and thawing cells.
CRFT liquid nitrogen free program cooling instrumentThe internal modules are replaceableDifferent modules can be selected based on the different freezing containers,Make the experiment no longer limited to freezing capacity and containersIt has a size of only 345 * 290 * 380mm and weighs 8.9kg, which is very compact and does not take up a lot of space in the laboratory. Its power consumption is also very low,Reduce long-term operating costsThe software interface of the CRFT liquid nitrogen free program cooling instrument is also relatively simple, and temperature settings and report exports can be achieved on the software. You can choose an uninterruptible power supply (UPS), and if there is a power failure, you can continue to supply power to complete the cycle operation.
The CRFT liquid nitrogen free program cooling device is mainly aimed at universities, research institutes, and other institutions, focusing on cutting-edge fields such as genetic engineering, molecular biology, tumor research, and stem cell research.
The CRFT liquid nitrogen free program cooling device is safe and easy to operate, avoiding the risks of hazardous chemical management and biological pollution hazards related to liquid nitrogen; The simplicity of operation lies in the preset programmed freezing curve (one click setting of cooling rate/temperature gradient/time parameters, etc.), fully automatic operation, elimination of manual intervention, and elimination of repeated cooling operations and integrated cell freezing and recovery operations required by traditional methods.
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Dichbio
Point into Biology
Diancheng Biotechnology is a company specializing in one-stop solutions for the fields of biology and medicine (formerly known as Hongke Biotechnology Division). Supported by more than a decade of technological accumulation and customer base in the fields of medicine, biology, and healthcare, we focus on providing one-stop solutions for pharmaceutical companies, biotechnology companies, medical institutions, universities, and research institutions, serving from basic research to sample analysis, and the entire lifecycle of drugs to ensure quality and safety.
The main business scope covers: pharmaceutical supply chain environmental monitoring, temperature verification, life science instruments, laboratory equipment, medical equipment, and GxP compliance consulting and services.
We have deep and long-term technical cooperation with world-class multinational corporations and instrument manufacturing centers, committed to providing customers withexcellentInnovative solutions. By continuously innovating and providing professional services, we help our clients ensure accurate, reliable, and 100% compliant monitoring, validation, and experimentation processes. Our clients include companies and institutions such as Roche, Pfizer, Sanofi, Baxter, Lanzhou Biotech, Sinovac, Fosun Pharma, Lonza, BeiGene, WuXi AppTec, West China Hospital, Xiangya Hospital, Peking University, Tsinghua University, etc.
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