The pulsating vacuum sterilization cabinet is a new generation of equipment in China suitable for sterilization and drying of clothing, dressings, metal instruments, culture media, and glassware. Adopting PLC, human-machine interface fully automatic control, automatic temperature recording, vacuum drying and other functions. Widely used for disinfection and sterilization of items in hospital supply rooms, operating rooms, pharmaceutical factories, laboratories, scientific research and other units.
The sterilization effect evaluation of pulsating vacuum sterilization cabinet can be carried out from the following aspects:
1. B-D test method:
♦ Principle: Before sterilization, a B-D test should be conducted on the empty pot every day. Evaluate the effectiveness of the vacuum system in a fire sterilizer by assessing the effectiveness of air removal in the vacuum pressure steam sterilizer.
♦ Operation: Use a test bag wrapped in degreased cotton cloth, with a weight generally controlled at around 5kg. Place a specialized B-D bottom layer near the cabinet door and exhaust port. Determine the effectiveness of the sterilizer vacuum system based on the discoloration of B-D test strips.
♦ Evaluation criteria: If the color of the B-D test paper is uniform and consistent, it indicates that the vacuum system of the sterilizer is normal and can perform sterilization operations.
2. Chemical indicator tape method:
♦ Principle: Chemical indicator tape can be used to evaluate the temperature and time of the sterilizer throughout the entire sterilization process. When the specific sterilization time and temperature reach a set standard, the stripes of the tape will change color.
♦ Operation: Stick chemical indicator tape onto each package of items to be sterilized, and preliminarily evaluate the sterilization effect by observing the discoloration of the tape.
♦ Evaluation criteria: The uniform discoloration of the tape indicates that the item has undergone corresponding sterilization treatment, but it does not directly indicate whether the sterilization effect is qualified.
3. Chemical indicator card method:
♦ Principle: The chemical indicator card can indicate the temperature of the base gas and monitor the specific duration of this temperature.
♦ Operation: Place the chemical indicator card in the center of the item package in the area with high sterilization difficulty, remove it after sterilization, and judge the sterilization effect based on the color change.
♦ Evaluation criteria: If the color of the indicator card turns black, it indicates that the sterilization effect has been qualified; The color of the indicator card is lighter than black, indicating that the sterilization effect is not qualified.
4. Biological monitoring method (not mentioned in the article, but is an important evaluation method):
♦ Principle: Using a heat sensitive biological indicator, the sterilization effect can be directly evaluated by measuring whether the microorganisms in the biological indicator have been completely killed.
♦ Operation: Place the biological indicator into the item to be sterilized, remove it after sterilization treatment, cultivate and observe the growth of microorganisms.
♦ Evaluation criteria: If all microorganisms in the biological indicator are killed, it indicates that the sterilization effect is qualified; If there is microbial growth, it indicates that the sterilization effect is not qualified.
5. Physical monitoring method:
♦ Principle: Evaluate whether the sterilization effect meets the expected standards by monitoring physical parameters such as temperature and pressure during the sterilization process.
♦ Operation: Use instruments such as thermometers and pressure gauges to monitor temperature, pressure, and other parameters during the process of fire and bacteria in real time.
♦ Evaluation criteria: If the temperature, pressure, and other parameters during the sterilization process meet the preset sterilization requirements and are maintained for a certain period of time, it indicates that the fire sterilization effect is qualified.
In summary, the evaluation of the fire sterilization effect of the pulsating vacuum fire sterilization cabinet should comprehensively adopt various methods such as B-D test method, chemical indicator tape method, chemical indicator card method, biological monitoring method, and physical monitoring method to ensure the reliability and safety of the sterilization effect.