Recently, the Shuangjia editor has summarized some common questions about ultrasonic cell disruptors that users are more concerned about, and provided corresponding answers here, hoping to bring some help to users and have a deeper understanding of ultrasonic disruptors.

1、 How to use the ultrasonic cell grinder correctly?

Put the material you want to crush into a beaker or test tube, place the ultrasonic probe into the crushed solution, set the working time and ultrasonic power of the ultrasonic generator, and note that there are two time settings, one is the vibration time and the other is the intermittent time. It should also be noted that a large amount of heat is generated during the crushing process, so it is generally reasonable to choose to crush under an ice bath.

2、 What are the components and structural characteristics of an ultrasonic cell grinder?

1. The ultrasonic cell grinder consists of three parts: an ultrasonic generator, a transducer component (transducer, amplitude rod), and a soundproof box.

2. The power of ultrasound is continuously adjustable from 0 to 100%.

3. There are two working modes to choose from: intermittent (duty cycle adjustable from 1% to 100%) and continuous.

4. The working time can be controlled by a digital timer, which automatically switches to standby mode when the predetermined time is reached.

5. Good equipment has multiple protection functions such as temperature protection control and overcurrent protection.

3、 What are the precautions for using an ultrasonic cell grinder?

1. The probe tip is 10-15mm away from the liquid surface;

2. The container for holding samples must be a glass container;

3. The ultrasonic probe should not touch the container wall and bottom;

4. The sample must be in aqueous phase rather than organic phase;

5. When using a microprobe, the amplitude adjustment should not exceed 70%.

4、 What are the common uses of ultrasonic cell disruptors?

1. The fragmentation, lysis, and extraction of cell structures, DNA/RNA extraction and pruning, protein extraction, chromatin immunoprecipitation, etc. in biological sciences;

2. The dispersion of nanomaterials, homogenization of soil, rock samples, disintegration, emulsification, homogenization, and fragmentation of particulate matter such as non-ferrous metals and rare earths in materials chemistry;

3. The dispersion, extraction, emulsification, homogenization, and accelerated reaction process of Chinese herbal medicines in the pharmaceutical industry;

4. The processing of samples in various fields such as chemical engineering, medicine, archaeology, geology, and oceanography.