Bailun 5-liter 10 liter glass cell bioreactor 1. Suitable cell culture medium

A suitable cell culture medium is one of the important conditions for in vitro cell growth and proliferation. The culture medium not only provides cellular nutrition and basic substances that promote cell growth and proliferation, but also provides a living environment for the growth and proliferation of cultured cells.

2. High quality serum

Currently, most synthetic culture media require the addition of serum. Serum is one of the important components in cell culture medium, containing various growth factors and other nutrients required for cell growth.

3. Aseptic and non-toxic cell culture environment

A sterile and non-toxic operating environment and culture environment are the primary conditions for ensuring successful cell culture in vitro. Cells cultured in vitro lack the ability to defend against microorganisms and toxins. Once contaminated by microorganisms or toxic substances, or if their own metabolites accumulate, it can lead to cell poisoning and death. Therefore, when cultivating cells in vitro, it is necessary to maintain a sterile and non-toxic environment for cell survival, and promptly remove cellular metabolites.

Bailun 10L glass cell bioreactorMade of glass material, easy to observe. Sterilized in place, simple and convenient,The failure rate is extremely low,Small footprint, low investment, short lead time) cell culture tool.

4. Constant cell growth temperature

Maintaining the vigorous growth of cultured cells requires a constant and suitable temperature. Low temperature egg whites fold and express correctly, with high activity. At higher temperatures, rapid bacterial growth can affect the stability of recombinant plasmids, resulting in the production of protein in the form of inclusion bodies and an increase in byproducts, which inhibit bacterial growth. Priority will be given to bacterial growth in the early stages, and the expression of the target product will be placed in * later on. Bailun BiologicalCellular bioreactorThrough intelligent PID temperature control and water circulation, with an accuracy of 0.1 degrees.

5. Appropriate temperature for cell growth

Gas is one of the essential conditions for mammalian cell culture and survival, and the required gases mainly include oxygen and carbon dioxide.Bailun cell bioreactorGas flowmeter configuration: air, oxygen, carbon dioxide, nitrogen. Multiple ventilation combinations are available for users to choose from, which can be associated with: DO/PH self-control, constant flow rate can be set, software automatically calculates cumulative amount, and deep layer gas can be freely switched.

Bailun100L stainless steel bioreactor