1、 Daily operation standards
1. Accurate control of environmental parameters
Temperature and pH adjustment: Maintain the optimal reaction conditions for enzymes (such as 50-70 ℃ for thermophilic enzymes), and pH fluctuations should be ≤± 0.2 to avoid enzyme inactivation.
Fluid stability: Constant flow rate and stirring speed (25-200 rpm) to reduce mechanical shock on immobilized enzyme carriers.
2. Pollution prevention management
Aseptic operation: High temperature sterilization (121 ℃, 30min) or chemical disinfection (0.5% sodium hypochlorite) before use, and a sterilization filter is installed at the feeding end.
Pollution monitoring: Microbial contamination is detected in each batch (such as ATP bioluminescence method), and any abnormalities are immediately stopped for processing.
2、 Core component maintenance
1. Enzyme carrier/immobilization system
Activity monitoring: Regularly test substrate conversion rate, and replace or regenerate the carrier when the activity decreases by more than 20%.
Carrier integrity check: Observe whether the microspheres/particles are fragmented (if the fragmentation rate is greater than 5%, replacement is required).
2. Sensor and Instrument Maintenance
DO/pH electrode: Replace the electrolyte and polarize for 6 hours every 3 months, and replace the membrane component in a timely manner when it is abnormal.
Flow calibration: Monthly verification of flow rate deviation (>± 5% needs to be recalibrated).
3、 Cleaning and regeneration program
1. Physical cleaning
Backflushing and rinsing: Rinse the pipeline daily with deionized water (pressure ≤ 0.1MPa) to remove residual substrates.
Membrane maintenance (if including separation membrane): Clean the air every week to prevent blockage, and start chemical cleaning when the flux decreases by more than 20%.
2. Chemical cleaning
Conventional cleaning: After each batch, use 0.1M NaOH or specialized enzyme cleaning agent to circulate for 30 minutes to remove protein deposits.
Deep regeneration: Soak every 10 batches using the acid-base alternation method (pH2 hydrochloric acid → pH12 sodium hydroxide) for 2 hours.
Sensitive enzyme protection: Lipase and other shear sensitive enzymes require the use of low shear stirring blades (such as anchor type).
4、 Key points for maintenance of special systems
1. Continuous flow microchannel reactor
Anti clogging: Install a<100 μ m filter at the feed end and monitor the pressure difference in real-time (Δ P>15% needs to be checked).
Material protection: Avoid cleaning glass/ceramic microchannels with strong oxidants to prevent corrosion.
2. Shutdown maintenance
Drain the residual liquid and inject inert gas (such as N ₂) to prevent oxidation.
If the immobilized cell system is shut down for more than 72 hours, protective solution (containing 5% glycerol buffer) should be injected.
5、 Periodic maintenance tasks
1. Monthly tasks: Sensor calibration, carrier integrity check.
2. Quarterly task: Comprehensive chemical cleaning (acid-base alternation+enzyme cleaning agent).
3. Annual task: Replacement of seals (O-rings), system performance validation (substrate conversion rate testing).
Key reminder: After strong acid/alkali cleaning, it is necessary to thoroughly rinse until neutral, as residual chemicals will irreversibly damage enzyme activity; Enzyme specific cleaning agents (such as protease complexes) have a removal efficiency of over 40% higher than ordinary cleaning agents for organic pollution.