Anaerobic cultivation systems are mainly used for the cultivation of microorganisms such as anaerobic bacteria, microaerophilic bacteria, facultative anaerobic bacteria, and CO2 loving bacteria that have high requirements for oxygen concentration or carbon dioxide concentration. They can be applied in fields such as biopharmaceuticals, food, inspection and testing, inspection and quarantine, agriculture, forestry, animal husbandry, and fishery, environmental protection, and scientific research.
Anaerobic cultivation systems typically consist of the following basic structures:
Box: It is the main body of the system, generally made of corrosion-resistant stainless steel and other materials, with good sealing performance to prevent external oxygen from entering. There are compartments or brackets inside the box for placing culture vessels such as petri dishes and test tubes.
Cultivation tank or anaerobic bag: It is the core part that provides an anaerobic environment. Cultivation tanks are usually sealed glass or stainless steel containers with gas inlet and outlet for controlling and replacing gases; Anaerobic bags are relatively simple equipment commonly used in small-scale experiments, making them easy to operate and move.
Gas control system: used to control the gas composition and pressure inside the incubator, including gas supply devices, gas flow control devices, and gas discharge devices, usually providing gases such as nitrogen, hydrogen, carbon dioxide, etc. to maintain an anaerobic environment.
• Temperature control system: composed of temperature sensors, heating devices, and automatic adjustment control systems, it can accurately monitor and adjust the temperature inside the box, providing suitable growth temperature for anaerobic microorganisms. Generally, the temperature can be adjusted within a certain range according to demand.
• Humidity control system: Some anaerobic cultivation systems are equipped with this system, which consists of humidity sensors, humidifiers, and dehumidification devices, to control the humidity inside the box and ensure stable humidity in the microbial growth environment.
• Operating room and sampling room: The operating room is equipped with gloves or sleeves, and experimenters can operate without damaging the anaerobic environment; The sampling room is used to safely transfer samples into and out of the incubator, preventing external air from entering.
Alarm system: capable of real-time monitoring of changes in parameters such as temperature, humidity, and gas concentration. Once an abnormality occurs, the system will automatically issue an alarm to remind operators to handle it in a timely manner.