Beginner's Quick Guide to Avoiding Pits on Ultra Clean Workbench

Gathering of treasures from the fields of biology, medicine, and microbiology! Incorrect use of the ultra clean workbench, sample contamination, and experimental overturning are common occurrencesToday I'll give you the nanny level guide that summarizes the pitfalls, so beginners can easily copy their homework without panic!First understand the core: it is not an ordinary workbench! The core of the ultra clean workbench is "creating a sterile environment", which filters the air through vertical laminar flow and blows it towards the table to protect the samples from contamination! But the key point is that it does not protect people! Do not use it when dealing with pathogenic bacteria and viruses, you need to switch to a biosafety cabinet! For example, it's OK to use it for E. coli cultivation, but not for COVID-19 experiment. Mixed use will cause big problems!

01Novices must learn the operation process（3Step by step, copy directly)

01Pre processing during startup (the key to determining the aseptic effect!)02Tips for avoiding pitfalls in experiments (beginners are most likely to make mistakes!) Item placement: Place the cleaning area (pipette, unopened culture medium) on the left side, the operating area (sample in use) in the middle, and the contaminated area (waste liquid tank, used straw) on the right side. Everything should be at least away from the air outlet15cmDon't block the airflow! Operation action: Insert your arm vertically from below the front of the workbench, move gently and gently without swinging your arm! For example, when holding a sample, moving slowly and swinging the arm quickly can disrupt laminar flow and bring in external bacteria. Prohibited behavior: It is strictly forbidden to stick your head in to look at the sample! Don't write experiment records or put your phone inside, and don't use open flames (which will damage the sterile laminar flow)03After the experiment, please put the contaminated waste into a special sealed bag and wipe the table with alcohol to keep the fan running10Minutes, ensure that residual pollutants are absorbed, then turn off the fan and finally turn on UV disinfection30During the disinfection period, no one should approach the workbench

02 90%People have stepped on these mines!

misconception1To save electricity, the fan only needs to be turned on for a few minutes and the laminar flow is unstable. A sterile environment has not been formed, and the sample must be contaminated! Minimum waiting for fan operation30minute

misconception2UV lamp24When the lamp is turned on for hours, its lifespan is limited8000When driving continuously for hours, it not only has no effect, but also produces harmful substances. Do not drive when people are present!

misconception3The filter never changes. The initial efficiency filter is cleaned monthly1Next time,HEPAFilter is inspected annually1Secondly, blockage will greatly reduce the sterile effect!

Old laboratory staff sincerely remind that the core of the ultra clean workbench isMaintain sterile laminar flow, all operations must revolve around this principle! Novices, don't be afraid of trouble. Just follow the process a few times and you'll be proficient, saving too much trouble compared to doing rework