What is it that an extruder with a larger pore size filter membrane is better than one with a smaller pore size filter membrane,

You can find the reasons from the following aspects

1. Large particle size, several times over 400nm and then over 200nm

2. What phospholipids are used? What is the extrusion temperature? Temperature below the phase transition temperature, strong rigidity of liposomes, and high extrusion pressure

3. What is the dosage of cholesterol in the prescription? The amount of cholesterol used can also affect the rigidity of liposomes, which in turn affects extrusion pressure

4. Blank liposomes or drug loaded liposomes? Generally, when drug loaded liposomes are extruded, the pressure is higher

5. If you really can't change the mechanical equipment, don't squeeze your hands. The extrusion pressure of 200nm requires 10-30 bar, and manual extrusion is quite difficult