Thermoelectric liquid chromatography is a powerful analytical technique that covers almost all fields that require separation and analysis, including synthetic product purity analysis and reaction process monitoring in the chemical field; Separation and analysis of biomolecules such as proteins, peptides, and nucleic acids in the field of biology; Analysis and monitoring of pollutants in environmental samples such as water bodies, soil, and atmosphere in the field of environment; Testing and analysis of food additives, nutritional components, pesticide residues, etc. in the food industry; And various links in the drug development and production process.

Precautions for using thermoelectric liquid chromatography technology:

1. Mobile phase management

-Filtration and Degassing: All mobile phases must be filtered through a membrane and thoroughly degassed to prevent particles from wearing the sealing ring or generating bubbles that could affect pressure stability.

-Compatibility control: Avoid mixing incompatible solvents (such as concentrated sulfuric acid and organic phases), and buffer salt solutions should not be left in the pump for a long time, otherwise crystals may precipitate and damage components.

2. Column protection

-PH range limitation: Strictly follow the pH tolerance range indicated on the chromatographic column to prevent hydrolysis or bond breakage of the stationary phase.

-Daily maintenance: When not in use for a long time, rinse with designated solvents and seal for storage; Regularly regenerate the column efficiency with low concentration organic solvents.

3. Safety operation standards

-High pressure protection: It is forbidden to directly touch the high-pressure pipeline interface during operation to prevent injury caused by liquid spraying.

-Chemical protection: Wear personal protective equipment such as gloves and goggles, especially when handling toxic or corrosive reagents.

4. Troubleshooting and emergency response

-Abnormal pressure handling: If the pressure is too high/too low, the pipeline should be checked section by section for blockage or leakage; In gradient elution mode, attention should be paid to the viscosity differences caused by changes in solvent mixing ratios.

-Emergency stop: Familiarize yourself with the location of the emergency stop button on the instrument. In case of sudden situations such as liquid leakage or fire, immediately cut off the power and take corresponding measures.

5. Data reliability assurance

-Environmental control: Maintain a constant laboratory temperature to avoid changes in the composition of the mobile phase caused by temperature differences.

-Record completeness: Detailed recording of experimental conditions (flow rate, column temperature, detection wavelength, etc.), sample information, and abnormal phenomena for easy traceability and reproduction of results.

6. Key points for instrument maintenance

-Regular calibration: including the verification of key parameters such as pump flow accuracy and detector sensitivity.

-Consumables replacement cycle: Timely replace vulnerable parts such as online filters and sealing gaskets to ensure system airtightness.