1.1.3Collection method:

1)Place a 5cm × 5cm sterilized specification plate on the surface of the object to be tested, soak the sampling swab head with sterile phosphate buffer solution prepared in the bag, apply it horizontally and vertically back and forth 5 times inside the specification plate, and then rotate the swab accordingly, continuously sampling 4 specification plates with an area of 100cm2;

2)Before use, gently shake and mix well to neutralize the eluent;

3) Insert the swab into the test tube and place the break point on the swab rod near the edge of the test tube mouth. Grasp the test tube with your left hand and the swab rod with your right hand, bend it about 45-60 degrees and break it, so that the swab head falls into the test tube. Discard the swab rod held in your right hand, tighten the test tube cap containing the swab head in a timely manner, and send it for testing in a timely manner; The operation diagram is as follows:

4）Small objects such as door handles with irregular shapes or less than 100 cm2 should be wiped with a moist swab head to collect the entire surface, without the need for a specification plate.

1.2 Elution: Shake the test tube containing the sampling swab head thoroughly on a vortex oscillator to elute the sample for at least 30 seconds, then immediately remove the swab head and discard it to ensure the reproducibility and accuracy of the results;

1.3 Vaccination:

1) Operating environment: At least 100000 level clean environment to prevent pollution, such as biosafety cabinets;

2）Take 1mL from 10mL of the eluted sample and the eluent, and perform colony counting using the pouring method (see Appendix A);

1.4 Cultivation: Cultivation: 35 ℃± 2 ℃, atmospheric environment, 48 hours;

1.5 Counting and Calculation

lWhen sampling 100 cm2, CFU/cm2=colony count x 10 * ÷ 100cm ²;

lWhen sampling<100 cm2, CFU/piece=colony count x 10 *;

Note: * Take 1mL of the neutralizing eluent from 10mL of the eluted sample and pour it for counting, i.e. dilute 10 times.

1.6 Standards and Interpretation

1.6.1The microbial limit results on the surface of the object after disinfection with disinfectant should meet the requirements of Table 1

Table 1. Surface hygiene standards for objects

lClass I environment refers to medical and treatment facilities that use clean air technology, divided into clean operating rooms and other clean areas;

lClass II environment, including non clean operating rooms, delivery rooms, catheterization rooms, hematology wards, burn wards and other protective isolation wards, intensive care units, neonatal rooms, etc;

lClass III environment, including mother and baby rooms, inspection and packaging sterilization areas, sterile item storage areas in disinfection supply centers, hemodialysis centers (rooms), and other general inpatient wards;

lClass IV environment, which is the general outpatient (emergency) clinic and its examination and treatment room, infectious diseases clinic and ward.

1.6.2Result explanation: If the calculated result meets the standard, it is qualified; On the contrary, it is unqualified.

2. Medical staff's hand hygiene check, hands disinfected with disinfectant (wearing gloves)

2.1 sampling

2.1.1Sampling timing: After practicing hand hygiene, sample before coming into contact with the patient or engaging in medical activities;

2.1.2Sampling method: Soak the sampling swab head in sterile phosphate buffer solution prepared in the bag and rub it back and forth on the curved surface of both fingers from finger to finger end twice (with one hand rubbing an area of about 30 cm2), then rotate the sampling swab head accordingly, insert the swab head into the test tube, and the break point of the swab is against the edge of the test tube mouth. Hold the test tube with your left hand and the swab rod with your right hand, bend it about 45-60 degrees, and break it. The swab head falls into the test tube, discard the swab rod held by your right hand, tighten the test tube cap containing the swab head in a timely manner, and send it for testing in a timely manner;

2.2 Elution: Shake the test tube containing the sampling swab head thoroughly on a vortex oscillator to elute the sample for at least 30 seconds, then immediately remove the swab head and discard it to ensure the reproducibility and accuracy of the results;

2.3 vaccinate

1）Operating environment: At least 100000 level clean environment to prevent pollution, such as biosafety cabinets;

2）Take 1mL from 10mL of the eluted sample and the eluent, and perform colony counting using the pouring method (see Appendix A);

2.4 Cultivation: 35 ℃± 2 ℃, atmospheric environment, 48 hours;

2.5 Counting and Calculation: CFU/cm2=Colony Count × 10 * ÷ (30 × 2) cm ².

Note: * Take 1mL of the neutralizing eluent from 10mL of the eluted sample and pour it for counting, i.e. dilute 10 times.

2.6 Standards and Interpretation

2.6.1Hand hygiene testing standards

After hand disinfection, the total number of bacterial colonies on the surface of medical staff's hands should be ≤ 10 CFU/cm2

lThe total bacterial count on the surface of medical staff's hands after surgical hand disinfection should be ≤ 5 CFU/cm2

lRefer to other standards used by your company.

2.6.2Result explanation: If the calculated result meets the standard, it is qualified; On the contrary, it is unqualified.

Limitations of Inspection Methods

This product is only used for washing samples collected from swab heads, neutralizing residual disinfectants, and cannot be used alone. It needs to be combined with a filter, filter cup, other equipment, reagents, and culture medium to achieve the intended use.

Product Performance Indicators

【 Precautions 】

1. Aseptic operation, pollution prevention; This product is intended for one-time use;

2. This product is only for professional use according to experimental operating procedures and is limited to laboratory professionals;

3. Before use, users should check the packaging and appearance of the liquid. The accompanying culture medium should be sterile and cannot be used if it is contaminated;

4. Do not use expired or damaged packaging products. They should be sterilized under high temperature and pressure before disposal.

Explanation of Identification

1. The provided graphics, symbols, and writing include: