Composite neutralization eluent（50mL）Instruction manual
Common name: Composite Neutralizing Eluent
English name:Compound neutralizing liquid
50 mL/
bottle
8001
Other items not provided but needed:
1）The agar medium used for membrane filtration film inoculation mainly includes tryptone soy agar（TSA）Plate, nutrient agar（NA）Tablet and therapeutic water specialist
usedR2AAgar agar plate;
Used for microbial limit testing of endoscopes, washing out residual bacteria in endoscopes, and neutralizing residual disinfectants. No bacterial growth function, not part of the culture medium.
【 Principle of Inspection 】
Quantitative sampling, utilizing various neutralizing agents contained in the product to neutralize residual disinfectants on the sample, in order to relieve the inhibition of microorganisms by residual disinfectants and facilitate residual microorganisms
The cultivation and resurrection of organisms can be detected to avoid missed detections.
Compound neutralizing agents contained in liquids can neutralize various disinfectants, including iodine, chlorine containing disinfectants, peroxides, quaternary ammonium (benzalkonium bromide), aldehydes, and biguanides (washing agents)
Bitai). Therefore, there is no need to consider matching with the type of disinfectant when using this product, and there is no difficulty in choosing.
[Appearance]
Opaque, milky white to amber in color, with slight precipitation after prolonged storage (does not affect filtration, should be shaken and mixed well before use to neutralize the eluent).
Storage conditions and expiration date
Avoid light, at room temperature（10~30℃), expiration date1 Year. After opening the test tube cap, it should be used immediately.
1)
After cleaning and disinfecting the endoscope4Within the hour, use the product to rinse and wash all recovered solution from the endoscopic tube;
2)
Sampling: Unscrew the tip cover of the reagent bottle, tilt the bottle tip and insert it into the disinfected and cleaned endoscope port, squeeze and inject neutralizing eluent appropriately, and inject all of it
Enter and use a suction filter cup at the lower end of the pipeline to fully recover the neutralized eluent that flows out, and promptly send it to the laboratory for testing; After sampling1 hComplete internal filtration, if unable to do so in a timely manner
Processing should be done in accordance with2-8Refrigerate at ℃ and store in24hInternal processing..
1. Sampling and inoculation culture of disinfected endoscopes:
1.1.
Before use, gently shake and mix well to neutralize the eluent;
1.2.
Sampling: Unscrew the tip cover of the reagent bottle and insert the bottle tip into the disinfected and cleaned endoscope at an angle（4hThe upper port of the internal sampling is used to squeeze and inject neutralizing eluent,
Inject all and fully recover the neutralized eluent flowing out at the lower end,1Send the samples to the laboratory for testing within hours. If they cannot be processed in a timely manner, they should be sent to the laboratory2-8℃ refrigerated and
In24hInternal processing;
1.3.
Sample processing and inoculation:
1)
Translate into English50mLThe eluent is filtered on a suction filter
2)
Remove the filter membrane from the suction cup in a clean environment of at least 100000 levels, and slowly attach the filter membrane to the surface of the agar plate from one side with the front facing upwards (be careful not to leave any air behind)
Bubble),36℃±1℃ incubator cultivation, atmospheric environment,48h,Colony count;
1.4.
Incubation:36±1℃, atmospheric environment,48hColony count.
2. Calculation of membrane filtration method results:
1)
Endoscopic monitoring: colony count x dilution factor (if diluted)=CFU/document
2)
Treatment water: colony count x dilution factor (if diluted) ÷50mL=CFU/mL
Note: Endoscopic microbial limit testing is not applicable to the pour method, as this method requires multiplying the bacterial count by the number of colonies during calculation50Multiplying, once there is1A single colony exceeds the standard.
——Medical equipment including endoscopes
1)
Highly hazardous medical equipment should be sterile;