Cy3 NHS EsterCy3-N-Hydroxysuccinimide ester

Product Information

Product Name	Specifications	store	Price (CNY)
Cy3 NHS Ester Cy3-N-hydroxysuccinimide ester	10mg	-Store in a dark and dry place at 20 ℃	2050.00

SupplierXi'an Kaixin Biotechnology Co., Ltd

Please clickwestAnkai New Biotechnology Co., LtdShop View

Please clickwestAnkai New Biotechnology Co., LtdShop View

Product description

Cyanine 3（Cy3）， It is a member of the anthocyanin fluorescein dye family and a chemically synthesized polyacetylene dye. At present, Cyanine series dyes are supplied in two isomers, one is non sulfonated anthocyanins and the other is sulfonated anthocyanins. The spectral properties of the two are almost identical, so most applications can be interchangeable, for example, both can be used to label biomolecules such as DNA or proteins. The main difference between the two is that sulfonated dyes are water-soluble and do not require the use of organic co solvents in the aqueous labeling reaction system. They also reduce the possibility of dye aggregation in water and enhance labeling efficiency.

This Cyanine 3 (Cy3) is supplied in the form of sulfonated dye and is easily soluble in water, Ex=555 nm，Em=565nm， It has the advantages of bright fluorescence, strong photostability, and pH insensitivity, and is suitable for labeling biological molecules such as peptides, proteins, and nucleic acids for analysis of target proteins through subsequent fluorescence imaging and other fluorescence biology methods.

This Cy3 NHS ester, which contains an N-hydroxysuccinimide activating group, can directly interact with amino groups (- NH) on biochemical molecules₂）The reaction forms stable amide bonds, mainly primary amines.

Product properties

English alias（English Synonym）	Cyanine 3, monosuccinimidyl ester
Molecular weight（Molecular Weight）	829.03
Appearance（Appearance）	Purple solid
Ex/Em	555nm/565nm
Solubility（Solubility）	Dissolve in DMSO, DMF, water
Structural type（Structure）

Transportation and storage methods

Ice pack transportation. -Dry at 20 ℃ and store away from light, with a shelf life of 1 year.

Precautions

1) The NHS ester group is highly sensitive to humidity and must be stored dry. The product needs to be allowed to warm up at room temperature for at least 20 minutes before use.

2) The hydrolysis of NHS ester groups occurs in a water-soluble buffer system, which is a competitive reaction. It is recommended to perform the linking reaction of primary amines for proteins/peptides (concentration: 1-1mg/ml) within the pH range of 7-9. Using a buffer system without amino groups, pH 7-9。 For example, 100 mM sodium phosphate, 150 mM sodium chloride, pH 7.5; 100mM Hepes, pH 7.5。 100 mM sodium carbonate/bicarbonate, pH 8-9; Or 100mM boric acid buffer, pH 8-9.

3) Do not use any buffer containing primary amines, such as Tris or glycine.

4) For your safety and health, please wear lab coats and disposable gloves when operating.

Usage (using)Cy3-NHS EsterLabeling antibodies)

【Attention】This method can be used for labeling peptides, proteins, or other molecules. It is necessary to adjust the appropriate labeling buffer system, sample concentration, dye concentration, and other parameters according to the specific experimental system.

1) Dissolve the antibody in a suitable buffer solution without amino groups (e.g. 100mM sodium phosphate, 150mM NaCl, pH 7.5) to achieve a concentration of 1-5 mg/ml. 【note】If there is residual Tris or glycine contamination in the antibody, it needs to be dialyzed or desalinated into a suitable buffer solution.

2) Before use, dissolve Cy3 NHS in an appropriate amount of deionized water or anhydrous DMSO to a molar concentration 400 times that of the antibody. For example, if the concentration of IgG to be labeled is 1mg/ml (eg 6.7 μ M), the concentration of Cy3 NHS after dissolution is 2.7mM; if the concentration of IgG to be labeled is 2.5mg/ml, the concentration of Cy3 NHS after dissolution is 6.75mM;

3) Add an appropriate amount of Cy3 NHS reagent to the antibody to achieve the expected labeling efficiency. 【note】When labeling antibodies or other proteins with Cy 3 NHS ester, the dye/protein ratio of the high brightness conjugate is 4-12, depending on the specific application. Excessive ratios can increase non-specific background, lose antibody specific binding, and lead to aggregation. Suggest optimizing the tagging ratio based on the application.

4) Incubate at room temperature for 60 minutes (or longer);

5) Use appropriate buffer solution for dialysis or desalination column to remove unreacted reagents;

6) Measure the absorbance values of Cy3 antibody at 280nm and 550nm, respectively;

7) Measure the labeling efficiency (DOL) and labeling concentration of Cy3. See the following formula.