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Zhengzhou Century Shuangke Experimental Instrument Co., Ltd

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Share the instructions for using the UV transmittance reflectance analyzer throughout the article
Date: 2023-07-05Read: 4
  UV transmittance reflectance analyzerHigh sensitivity, easy to use, especially the 302NM wavelength has less destructive effect on the sample, so it is the updated product of the UV analyzer. The multi-functional UV analyzer uses a fully enclosed high-quality metal dark box, which is easy to use and operate without operating in the dark room. The instrument can be installed with a digital camera, which is easy to take pictures, and is suitable for DNA, RNA gel, protein gel; Thin layer chromatography is an analytical instrument used in teaching and research institutions such as molecular biology research and forensic identification.
Instructions for using the UV transmittance reflectance analyzer:
(1) Analyze and observe samples
1. Connect the power cord
In case of gel observation, it is recommended to select the wavelength of 302nm. At this time, press the wavelength selector switch 302nm to adjust the intensity of ultraviolet light, adjust the high intensity, observe the medium intensity to prepare gel samples, and adjust the low intensity ultraviolet light source for preparation, observation, cutting and other operations to reduce the damage of ultraviolet light to DNA. At this time, turn on the wavelength switch 365nm or 254nm. After selecting the appropriate wavelength, you can observe, analyze and cut the gel. 2. Place the electrophoretic gel sample or thin layer or paper layer sample in the center of the instrument, turn on the power supply, and make the fluorescence zui from the sample clear and bright. Usually, the closer the reflected ultraviolet is to the sample, the stronger the fluorescence. It takes about three minutes to turn on the device, which is the perfect time for observation and photography. Don't miss it.
3. When analyzing gel samples, the UV intensity can also be adjusted according to specific experimental needs. Simply rotate the light intensity knob to select the appropriate UV intensity, ranging from 5% to 100%. 4. If slicing is required, insert both hands into the rubber cutting ports on both sides to proceed. 5. UV fluorescent plate conversion: put the fluorescent plate directly on the UV light box to achieve switching between UV light and visible light, which is suitable for protein gel analysis.
(2) Take photos
1. Install the camera stand and fixing screws. The camera only needs to be equipped with a color filter
2. Place the camera lens into the circular hole in the middle of the camera mount, and take photos when the camera is stable. Attention: Adjusting the camera lens should be prepared in advance before taking timely shots. To ensure stability during shooting, use a shutter cable or selfie device.
(3) Attention:
① Before use, remove the protective film on the UV filter.
② Prevent the electrophoresis solution from contaminating the UV filter.
③ Before placing the film, the UV light switch should be turned off to prevent UV radiation from leaking and burning the human body.
④ After observation, the power switch should be turned off promptly to extend the lifespan of the UV lamp.
⑤ If possible, please regularly clean the UV filter with anhydrous ethanol to prevent mold growth.
⑥ It is strictly prohibited to directly contact the UV filter with metal objects or other hard objects to avoid scratching the UV filter.