Chromaster Ultra Rs Ultra High Performance Liquid Chromatography System

NegotiableUpdate on 03/01

Model

Nature of the Manufacturer: Producers

Product Category

Place of Origin

Online Consult

Overview

A powerful assistant for new discoveries and research. The future is born from this! Return to the starting point, rethink, and depict the future from scratch. With advanced technology, achieve high resolution and sensitivity. We boldly explore and create a brand new Chromaster Ultra Rs. The future of UHPLC will start from here.

Product Details

characteristic

140 MPa system pressure resistance (Hitachi High Tech Survey of Domestic Sales Models in Japan, July 2013) to help you achieve high-resolution analysis

UHPLC with a theoretical tray count of up to 50000 uses a high resolution chromatography column (LaChromUltra II) to help you achieve high-resolution analysis

Total reflection capillary flow cell, helping you achieve both high sensitivity and high resolution analysis simultaneously

Analysis system that combines HPLC and UHPLC

MEM chromatography column connector (with a pressure resistance of up to 140 MPa, optional) and the system's built-in waste liquid tank further improve operability

Support analysis from UHPLC to HPLC

140 MPa， Excellent pressure resistance performance

The binary pump adopts the new control technology LBT (Liquid Beat Technology) control technology, which enables stable liquid delivery from the low pressure zone to the high pressure zone of 140 MPa. For gradient analysis in high-pressure areas, well reproducible analysis results can also be obtained.
In addition, the extremely small system volume structure improves gradient responsiveness and strongly supports ultra high speed analysis.

Improved the stability of liquid delivery and achieved extensive analysis from UHPLC to HPLC

Using LBT control technology, the solvent volume elasticity coefficient is corrected, and the stability of liquid delivery is not affected by the liquid delivery pressure and solvent type.
Stable analysis results can also be obtained when using 5 µ m chromatography columns, 3 µ m chromatography columns, and 1.9 µ m chromatography columns for analysis.

[Conditions]
Eluent	20 mM KH₂PO₄/CH₃CN＝95/5
Detection	274 nm

High-resolution analysis

Achieve higher separation degree

UHPLC is considered difficult to perform high-resolution analysis of substances and impurities related to synthetic compounds such as drugs and chemical synthesis products.
The newly developed UHPLC uses a high separation chromatography column LaChromUltra II, coupled with sufficient system pressure tolerance of 140 MPa, to achieve high-resolution and high-efficiency analysis.

High resolution analysis of substances (impurities)

A separation model sample using erythromycin (a fourteen membered ring macrolide antibiotic) as the main component and related substances was compared using HPLC chromatography column and UHPLC chromatography column.

HPLC column [Conditions]
Column	LaChrom II C18（5 µm） 4.6 mm I.D.×150 mm L.
Flow rate	1.0 mL/min
Eluent	20 mmol/L Phosphate Buffer/CH₃CN/CH₃OH＝45/40/15
Detection	DAD 210 nm

UHPLC column [Conditions]
Column	LaChromUltra II C18（1.9 µm） 3.0 mm I.D.×250 mm L.
Flow rate	0.710 mL/min
Eluent	20 mmol/L Phosphate Buffer/CH₃CN/CH₃OH＝45/40/15
Detection	DAD 210 nm

By using the newly developed UHPLC with a high separation chromatography column, better separation results were obtained.

High sensitivity analysis

Never let go of hidden risks

The demand for high-sensitivity analysis of harmful substances and genotoxic impurities is increasing day by day. The diode array detector using a total reflection capillary flow cell can meet the needs of higher sensitivity analysis. In addition, a high-sensitivity flow cell with an optical path length of 65 mm is also provided.
And combined with the double helix mixer and the reduction of sample residue, it supports high-sensitivity analysis.

Total reflection capillary high-sensitivity flow cell

The new optical system of diode array detector (DAD) effectively reduces noise and drift, ensuring high sensitivity analysis results. By using (optional) a high-sensitivity flow cell (optical path: 65 mm), higher sensitivity analysis can be achieved.
The sensitivity is approximately 10 times that of our previous model (LaChromUltra), making it suitable for samples that require high-sensitivity analysis of substances (impurities), genotoxic impurities, and other related substances. It can be flexibly used for impurity spectrum control in the initial stages of raw materials, intermediates, and raw materials of drugs and chemical synthesis products.

The quartz glass capillary is used in the flow path of the flow cell, and the total reflection at the capillary interface is utilized to successfully reduce the loss of transmitted light in the flow cell.
Therefore, even if the optical path length of the flow cell is extended, the same baseline performance as before can be ensured, thereby achieving high sensitivity analysis.

twin-spiral mixer

The binary pump is equipped with a new double helix mixer with microfluidic engineering as standard.
Under the premise of low capacity, high mixing performance has been achieved, so extremely stable baselines can be obtained in gradient analysis, achieving higher sensitivity analysis.

Construction of Double Spiral Mixer
The flow path inside the mixer undergoes repeated branching and merging to achieve efficient solvent mixing. (Mixer capacity: 55 µ L)

[Conditions]
Column	LaChromUltra II C18（1.9 µm） 2.0 mm I.D.×50 mm L.
Eluent	A）0.1％ TFA/H₂O（v/v） B）0.1％ TFA/CH₃CN（v/v） A/B＝50/50
Flow rate	0.500 mL/min
Detection	UV 214 nm

Operability

easy to operate

Daily maintenance and consumable replacement can be carried out from the front of the instrument, improving operability.
For example, the sealing ring of the plunger rod of the pump, the valve core of the one-way valve, the sealing ring of the injection port of the automatic sampler, the replacement of the detector light and flow cell, etc., all use the recognized instrument front operation characteristics of Hitachi HPLC.

chromatographic column

LaChromUltra II series
High tolerance and high pressure resistance performance, achieving high separation degree

The organic-inorganic composite silicone material used in LaChromUltra II has better physical and chemical resistance than previous silicone particles. To maximize the high pressure resistance performance of UHPLC, a chromatography column with a particle size of 1.9 µ m and a length of 250 mm has been added, which can not only perform ultra high speed analysis but also high separation analysis, thus expanding the application of UHPLC.

Module product line

DAD (diode array detector) system
UV-VIS system

application

Introduce measurement examples of high-performance liquid chromatography in various fields.

Basic Course on High Performance Liquid Chromatography

Introduce the basic knowledge of high-performance liquid chromatography, including the principles and system composition of high-performance liquid chromatography, as well as the characteristics of various detection methods.