Microbial liquid strain fermentation tank

NegotiableUpdate on 01/08

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Overview

Microbial liquid fermentation tanks are divided into solid fermentation tanks and liquid fermentation tanks. Fermentation tanks are divided into seed tanks (strain fermentation tanks) and fermentation tanks based on their volume and purpose. Fermentation tanks are divided into ventilated fermentation tanks and anaerobic fermentation tanks according to different aerobic types. Fermentation tanks, seed tanks, available in carbon steel, stainless steel, high nickel based alloys, etc., are mainly used for biochemical fermentation production. Industries such as the production of monosodium glutamate, citric acid, enzyme preparations, solvents, pharmaceuticals, and food.

Product Details

Microbial liquid strain fermentation tank

Microbial liquid strain fermentation tankOverview

Common fermentation tanks are divided into solid fermentation tanks and liquid fermentation tanks. Fermentation tanks are divided into seed tanks (strain fermentation tanks) and fermentation tanks based on their volume and purpose. Fermentation tanks are divided into ventilated fermentation tanks and anaerobic fermentation tanks according to different aerobic types. Fermentation tanks, seed tanks, available in carbon steel, stainless steel, high nickel based alloys, etc., are mainly used for biochemical fermentation production. Industries such as the production of monosodium glutamate, citric acid, enzyme preparations, solvents, pharmaceuticals, and food.

1、 Basic conditions of fermentation tank

Mechanical stirring fermentation tank is one of the commonly used types in fermentation factories. It utilizes the function of a mechanical stirrer to thoroughly mix air and mash, promoting the dissolution of oxygen in the mash to ensure the supply of oxygen needed for microbial growth, reproduction, and fermentation. Basic requirements for using fermentation tanks:

1The fermentation tank should have a suitable aspect ratio. The ratio of height to diameter of a fermentation tank is generally1.7~4The longer the can body, the higher the utilization rate of oxygen.

2. Fermentation tanks can withstand a certain amount of pressure. Due to the pressure (air pressure and hydraulic pressure) and temperature inside the fermentation tank during disinfection and normal operation, each component of the tank must have a certain strength and be able to withstand a certain amount of pressure.

3. The stirring and ventilation device of the fermentation tank ensures sufficient mixing of gas and liquid, ensuring the necessary dissolved oxygen in the fermentation broth.

4. The fermentation tank should have sufficient cooling area. The microbial growth and metabolism process releases a large amount of heat, and in order to control the temperature required at different stages of the fermentation process, sufficient cooling area should be installed.

5. The fermentation tank should minimize dead corners as much as possible to avoid the accumulation of dirt and stains. Sterilization should be thorough to prevent bacterial contamination.

6. The shaft seal of the mixer should be tight to minimize leakage.

2、 Size ratio of fermentation tank body

The dimensions of each part of the tank have a certain proportion, and the ratio of the height to the diameter of the tank is generally around 1.7 to 4 times.

Fermentation tanks are usually equipped with two sets of agitators, with a spacing S of approximately three times the diameter of the agitators.

For large fermentation tanks and those with high liquid depth HL, three or more sets of agitators can be installed.

The bottom set of agitators is usually closer to the outlet of the air duct, and the distance C from the bottom of the tank is generally equal to the diameter of the agitatorDiHowever, it should not be less than0.8DiOtherwise, it will affect the circulation of the liquid.

3、 Structure of fermentation tank

The aerobic mechanical agitation fermentation tank is a sealed pressure equipment, with main components including the tank body, agitator, shaft seal, aerator, coupling, intermediate bearing, air blowing tube (or air injector), baffle, cooling device, manhole, and pipeline.

The system consists of fermentation tanks, air treatment systems, steam purification systems, electrical control systems, constant temperature systems and pipelines, valves, auxiliary systems, sensors and primary instrument systems, lower computer control systems (on-site PLC control system, secondary instruments), upper computer systems, and benches.

Technical Specifications

Nominal volume	5~3000L
Liquid loading coefficient	65-80%,best70%
Tank configuration	Stainless steel SUS316L/SUS304material Jacket temperature control and optimized flow design; Internal surface polishing, Ra≤0.6μmExternal surface polishing or matte treatment; Large angle long strip mirror, pHElectrodeDOThe electrode and temperature electrode interfaces are complete; The can lid is equipped with a flame inoculation port and a backup feeding interface, etc
mixing system	Top entry mechanical mixing system; Aseptic machine sealing system; DC mixing motor (10L)And below) or AC motors; Standard configuration 2Layer flat impeller, first stage high-efficiency defoamer blade; There are also options for inclined blades, curved blades, axial flow blades, etc; Adjustable speed, digital setting speed control
Structural method	Floor standing frame structure, space saving, universal casters, easy to operate
ventilation system	Deep ventilation, all stainless steel pipeline; Glass rotor flowmeter displays pressuredisplayManually adjust the flow rate with a valve; Efficient sterilization filter equipped with stainless steel casing, with a filtration accuracy of 0.01 μ m； External compressed air is required, equipped with high-quality water removal and pressure stabilization devices, to provide continuous and stable gas supply; Optional mass flow controller for automatic control of air flow
Tank pressure control	The top exhaust port is equipped with a pointer pressure gauge to display tank pressure, and a stainless steel valve for manual adjustment; Optional imported pressure transmitter and automatic regulating valve for automatic control of tank pressure
temperature control	Online detection, digital setting, automatic/Manual control for free switching; German Pt-100Temperature probes and digital temperature instruments; Electric heating constant temperature water tank and automatic temperature control of external cooling water
Phcontrol	Online detection, 1Road or2The peristaltic pump automatically adjusts the flow of alkali or acid solution; Imported Mettler (Hamilton) pHElectrode, capable of high-temperature steam disinfection
Dissolved oxygen (Do)	Online detection, range 0-100%or0-200%； Imported Mettler (Hamilton) DOElectrode, capable of high-temperature steam disinfection; Optional linkage control with speed and ventilation volume (to be specified when ordering) Online detection, range 0-100% or 0-200%;
Supplement control	Standard configuration 1road/2Multi channel peristaltic pump with proportional flow and replenishment, cumulative display and recording of flow and replenishment volume
Defoaming method	Conductive foam electrode, foam abnormal condition automatic alarm; Standard configuration 1Road peristaltic pump automatic/Manual addition of defoamer, cumulative display and recording of added amount
control system	Application of industrial grade MC bioControl system, using SiemensPLCcontroller; 32A true color touch screen operation interface with complete functions and stable operation; Another AOptional simplified instrument control system
Sterilization method	In situ sterilization, manual control, requires external saturated steam connection
Optional accessories	1.Multi channel replenishment 2.Fully automatic sterilization 3.Supplement weighing 4.Tank weighing 5.Oxygen enriched bypass 6.Online detection of oxidation-reduction potential 7.Automatic detection and control of air flow rate 8.exhaustCH4，O2TheCO2Content online detection 9.Online detection of methanol (ethanol) content 10.Online detection of glucose and specific amino acids
Features of the equipment	In situ sterilization, safe and reliable, accurate concentration of culture medium; No transmission leakage risk; Clear observation through a wide-angle longitudinal mirror; Multiple and reliable vaccination methods are available (including standard flame vaccination port, differential pressure vaccination port, and other optional forms); The floor standing tank shape is beautiful and elegant; Easy to operate and disassemble; Special specifications can be customized.

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Microbial fermentation tank