NEPA21 Efficient Gene Transfection System

NegotiableUpdate on 02/13

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Overview

NEPA21 Efficient Gene Transfection System

Product Details

　 After close proximity30With the development and innovation of the year, electroporation has become an indispensable technical means in the field of gene functional research. As the exclusive agent in China, Huayuexing Instrument Co., Ltd. recommends Japan to youNEPA GENEThe company's instruments:

NEPA21Efficient gene transfection system

------Suitable for in vitro use(In Vitro)And living beings(In Vivo)

NEPA GENE specializes in the research and production of cell transfection and fusion devices. Its CUY21 series transfection devices have long been renowned in the research field and have been cited in hundreds of articles, including those from high-level journals such as Nature, Cell, PNAS, Genes&Development, etc.

In 2011, NEPA GENE launched a new generation of versatile NEPA21 efficient gene transfection system. Compared with traditional electroporation instruments, NEPA21 adopts a newly designed electroporation program, which is particularly suitable for transfection of difficult to transfect cells, ex vivo tissues, or live animals. Combined with its unique Voltage Decay design, NEPA21 can achieve high transfection efficiency while improving cell survival rate.

Main features:

A brand new four step electric conversion program
Specially suitable for transfection of difficult to transfect cells, ex vivo tissues, or live animals
High transfection efficiency and high cell survival rate
The parameters of the electric conversion program are visible, adjustable, and widely applicable
No need for special transfection reagent kit assistance, low operation and maintenance costs

New four step electric conversion program:

Step 1: Electric perforation mode

High voltage, short duration, multiple pulses, voltage decay

Effectively forming small pores on the cell membrane with minimal damage to the cells

Step 2: Reverse electroporation mode

Used for transfection of in situ adherent cells/tissues, effectively improving perforation rate!

Step 3: Gene Introduction Mode

Low voltage, long duration, multiple pulses, voltage decay

Help target molecules (such as DNA or RNA) enter the cell with minimal damage to the cell

Step 4: Reverse Import Mode

Unique design! Effectively improve import efficiency!

Application scope:

♦ Suspension transfection

Scope of application: Primary cells, stem cells, and various difficult to transfect cells (such as nerve cells, immune cells, and blood cells)

♦ Adhesive transfection

Scope of application: It can directly transfect adherent cells, eliminating the steps of cell digestion and reattachment (which is important for improving the survival rate of certain types of cells)!

♦ Ex vivo tissue transfection

Scope of application: Tissue sections, brain sections, organs, embryos, etc

♦ In vivo transfection

Scope of application: brain, retina, muscles, skin, liver, kidneys, testes, ovaries, etc

Partial references:

Cell transfection instrument (NEPA21, CUY21 series):

Barnabe-Heider et al.Genetic manipulation of adult mouse neurogenic niches by in vivo electroporation. Nature Methods,2008 Feb; 5(2):189-96.
Shibata MA, et al.Combination therapy with short interfering RNA vectors against VEGF-C and VEGF-A suppresses lymph node and lung metastasis in a mouse immunocompetent mammary cancer model.Cancer Gene Ther.2008 Dec; 15(12):776-86.
Limura T and Pourquie O.Collinear activation of Hoxb genes during gastrulation is linked to mesoderm cell ingression.Nature,2006 Aug 3; 442(7102):568-71.
Sanada K and Tsai LH.G Protein betagamma Subunits and AGS3 Control Spindle Orientation and Asymmetric Cell Fate of Cerebral Cortical Progenitors.Cell,2005 Jul 15; 122(1):119-31
Ladher RK et al.FGF8 initiates inner ear induction in chick and mouse. Genes and Development,2005 Mar 1; 19(5):603-13.

For more literature or product information, please visit the English website: http://www.nepagene.jp/E/Eindex.htm Or our company website http://www.huayueco.com/show12345.php?lanmuid=3&id=304

Do you want to learn about live transfection techniques? The world's first scientist to report the use of electroporation for in vivo transfection of mouse embryonic brains (Neuroscience. 2001; 103 (4): 865-72), Dr. H Tabata from Keio University personally demonstrated the method of in vivo embryo transfection for you. Please click to watch the video: http://video.sina.com.cn/v/b/63556016-2484835144.html

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