Pierce ? IP fragmentation buffer

Characteristics of IP fragmentation buffer:

Optimized to be compatible with various precipitation and pull-out assays
• Compatible with various protein assays, reporter gene assays, and * * testing programs
Ready made formula that can effectively extract cytoplasm, cell membrane, and nuclear proteins
A mild formula can help maintain protein complexes for co precipitation
• Will not release genomic DNA that can cause high viscosity of the sample

IP lysis buffer is a mammalian whole cell lysis reagent based on an improved RIPA buffer formula (without SDS). This medium strength lysis buffer can efficiently dissolve cellular proteins, but does not release genomic DNA or disrupt protein complexes like typical RIPA buffer. Pierce IP lysis buffer is specially formulated and can be used for various pull-out assays and * * precipitation detection.

Pierce IP lysis buffer can effectively lyse cultured mammalian cells from well plate cells and cells granulated in suspension culture medium. This lysis buffer has been optimized for various pull-out assays and precipitation detection, and is compatible with many other applications, including Thermo Scientific Pierce BCA and 660 nm protein assays, protein purification, and various detection methods such as ELISA and Western blotting.

Pierce IP lysis buffer consists of 25 mM Tris HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% NP-40, and 5% glycerol. This buffer does not contain protease or phosphatase inhibitors; But if necessary, various inhibitors can be added before use, such as Thermo Scientific Halt protease inhibitor mixture or phosphatase inhibitor mixture, to prevent protein hydrolysis and maintain protein phosphorylation.