Speaking ofCationic lipid material DC-CHOLFriends should not be unfamiliar with it. In this issue, AVT editor will share with you the cationic lipid DC-CHOLRNA liposomesWhat is the application in.

What kind of phospholipid is cationic lipid DC-CHOL?

Supplied by AVTDC Cholesterol Products DC CHOL, is a cationic liposome material for injection, with high purity, abbreviated as DC-CHO in English, CAS number 166023-21-8. This pharmaceutical excipient needs to be stored at -20 ℃ and is commonly used in the preparation of cationic liposomes. The specific product information is summarized as follows:

1 Chinese name: DC cholesterol (for injection)

2 Product Name: DC-CHO

3 Chemical name: 3 β - [N - (N ', N' - dimethylaminoethyl) carbamoyl] cholesterol hydrochloride

Molecular formula: C₃₂H₅₇ClN₂O₂

Manufacturer: Avito (Shanghai) Pharmaceutical Technology Co., Ltd

6 CAS number: 166023-21-8

Level 7: Reagent Grade

8 Purpose: Liposomes

9 Structural formula:

10 Characteristics: White powder

11 Purity: 98%

12 Solubility: soluble in water (partially miscible), easily soluble in methanol, ethanol, chloroform DMSO。

13 Molecular weight: 537.26

14 Storage conditions: -20 ℃

15 Precautions: Avoid contact with strong acids, bases, and oxidizing substances

What is the application of DC-CHOL in RNA liposomes?

RNARNAinterference (RNAi) is a rapidly developing research technique that may have promising applications in inhibiting tumors, antiviral therapy, and treating functional disorders in the body. But like antisense nucleic acids, how to remove small interfering RNA (siRNA),_SThe safe and effective delivery of RNA to target organs and cells is a challenge that hinders the development of this technology. The main obstacle in the development of RNA interference technology is how to effectively deliver small interfering RNAs to specific target cells in the body.

At present, nucleic acid drug delivery systems can be divided into two categories: viral vector systems and non viral systems. Liposome mediated nucleic acid drug delivery is the main method of non viral delivery systems.Cationic liposome DC Chol/DOPEIt has been successfully used for in vivo delivery of DNA drugs. DC Chol/DOPE is the first liposome to enter clinical trials, which can safely and effectively deliver DNA drugs, and patients have good tolerance to it.

by comparisonDC-Chol/DOPEThe transfection efficiency and toxicity of commercial transfection reagent Lpofectam ine 2000, which can efficiently mediate sRNA transfection in HeLa cells, were compared with commercial transfection reagent Lipofectamine 2000. The results showed that DC Chol/DOPE mediated sRNA transfection in Hela cells was the same as that of commercial transfection reagent Lipofectamine 2000Gao XiaoAnd its toxicity to cells is much smaller, which is expected to be further used for in vivo delivery experiments of sRNA.

Cationic lipid DC-CHOLInRNA liposomesThat's all for the application introduction!

Part of the content of this article is excerpted from "DC-CHOL/DOPE mediated sRNA effects on H eLa cellsGao XiaoTransfection "by authors Dang Ying, Wang Yingxiong, Zhu Xudong, Guo Weiwei, Wang Yuxin, Huang Peitang