How long is the shelf life of an ELISA kit? The shelf life of an ELISA kit is usually 6 months, but the specific duration depends on the date indicated on the outer packaging of the kit. The standard shelf life of unopened reagent kits is generally 6 months when stored in the dark for 2-8 hours. Some special components (such as freeze-dried standards) can extend their storage time under -80 conditions. 2. Shelf life After opening, liquid reagents (such as enzyme conjugates and chromogenic substrates) should be stored for 4 days and used up within 1 month. The pre coated enzyme label plate can be disassembled for use, and the remaining Flat noodles need to be sealed and stored with desiccant. 3. Factors affecting shelf life Storage conditions: Temperature fluctuations or repeated freeze-thaw cycles can shorten the shelf life. Component type: HRP markers are sensitive to repeated freeze-thaw cycles, and it is recommended to package and store them separately. Pollution risk: If there is turbidity, sedimentation or color change after opening, it should be stopped immediately. 4. The risk of using reagents beyond their shelf life may lead to false negative/positive results, and even if the appearance is normal, it may affect the accuracy of the test due to decreased activity. 5. Special cases have shown that some antibodies can still maintain their activity after 12-26 years of refrigeration, but experimental verification is required. Note: The above is for reference only.