Problem 1: Weak or undetectable Raman signal

First, check if the laser is turned on normally and if the output power meets the standard, and confirm that the laser path is unobstructed; Ensure that the sample is correctly placed in the focal position, especially for trace samples, the XYZ three-dimensional displacement table needs to be finely adjusted; Check if the numerical aperture (NA) of the microscope objective matches, as high NA objectives can significantly improve signal collection efficiency; Confirm whether the slit of the spectrometer is open and whether the cooling of the detector (such as CCD) is in place to avoid dark noise flooding the signal due to high temperature.

Problem 2: The fluorescence background is too strong, masking the Raman peak

Although resonance Raman can enhance the target signal, it may also excite the sample's own fluorescence. The solution includes: trying to change the excitation wavelength and avoid the strong absorption band of the sample; Using time gating or confocal techniques to suppress long-lived fluorescence; For solid samples, compression or dilution can be attempted to reduce concentration; Use baseline correction algorithms (such as polynomial fitting) to subtract background in post-processing.

Problem 3: Spectral appearance of "ghost peaks" or wavelength calibration drift

Ghost peaks often originate from light path pollution or stray light. Clean the objective lens, filter, and sample surface dust; Check whether the laser filter and notch filter are aged or misaligned; Using standard samples (such as 520cm silicon wafer)?? Regularly calibrate the wavelength of the peak, and if the deviation is severe, contact the manufacturer to realign the optical system.

Problem 4: Unstable laser power or local overheating of the sample

High power lasers can easily lead to sample degradation or carbonization. Neutral density filters should be used to adjust the incident power to a low effective level; For thermal sensitive samples, use fast scanning or reduce integration time; Ensure that the sample has good thermal conductivity, or use a water-cooled sample stage.

Problem 5: Software cannot control or data collection is interrupted

Check if the connection cable (USB/Ethernet) between the instrument and the computer is loose; Restart the control software and host; Confirm if the driver program has been updated; Avoid running multiple high occupancy programs simultaneously. If there are frequent disconnections, it may be a hardware interface failure.