1、 Preparation before system startup
1. Environmental and equipment inspection
Ensure that the surrounding environment of the equipment is dry and clean, and that all components (such as storage tanks, deactivation tanks, cooling tanks, etc.) are securely installed without leaks.
Check whether the pipeline connections, valve status, and sensors (thermometers, pressure gauges) are normal.
2. Parameter setting and drug preparation
Select the inactivation method (chemical or thermal sterilization) based on the characteristics of the wastewater, and configure the required chemicals (such as sodium hypochlorite, ozone, etc.).
Set parameters such as temperature, pressure, and processing time in the control system (for example, thermal sterilization needs to reach 121 ℃ for 30 minutes).
2、 Wastewater pretreatment and dosing
1. Wastewater collection and regulation
Introduce biological wastewater into a storage tank and monitor the capacity through a level gauge to avoid overflow.
Adjust the pH value (such as using acid-base neutralization) and temperature of the wastewater to improve the subsequent inactivation efficiency.
2. Drug administration
If chemical inactivation is used, disinfectant (such as sodium hypochlorite solution, ozone) should be accurately added through a dosing pump to control the concentration within the range of 5-10mg/L.
3、 Core stage of inactivation treatment
1. Thermal sterilization operation
Pump the wastewater into the inactivation tank, start the steam injector to heat it to the set temperature (such as 121 ℃), and monitor the inactivation effect in real time through the temperature measurement system.
Maintain a high temperature and high pressure environment for a predetermined period of time to ensure pathogen DNA/RNA fragmentation and microbial protein denaturation.
2. Chemical inactivation operation
If using chemical methods, mix the reagent with wastewater through a mixer to ensure sufficient contact and reaction.
Monitor conductivity or residual chlorine concentration to verify that inactivation meets standards.
4、 Cooling and waste liquid discharge
1. Cooling treatment
The inactivated high-temperature wastewater enters the cooling tank and is rapidly cooled to a safe temperature through jacket circulating cooling water (such as 15-25 ℃).
The liquid level and temperature of the cooling tank are fed back in real-time to the control system by sensors.
2. Waste liquid discharge and verification
The cooled wastewater is transported to the sewage treatment system through the discharge pipeline, and before discharge, samples need to be taken to test the inactivation effect (such as microbial residue).
If a continuous system is used, PLC will automatically control the discharge flow and batch switching.
5、 System shutdown and maintenance
1. Shutdown operation
Close the feed pump, mixer, and steam valve in sequence, and empty the residual waste liquid in the tank.
Use cleaning water (including acidic and alkaline solution) to perform CIP cleaning on the tank and pipelines to avoid biofilm residue.
2. Daily maintenance
Regularly replace filter components (such as sterilization filters), calibrate sensors, and check the status of seals and valves.
Record operational data (temperature curve, processing capacity) and backup it to the central control system, supporting quality traceability.
3. Key precautions
Safety protection: Wear protective clothing, masks, and gloves during operation to avoid contact with inactivating agents or high-temperature steam.
Emergency response: In case of power outage or equipment failure, immediately turn off the power and empty the wastewater in the tank to prevent pollution from spreading.
Compliance verification: Regularly test the inactivation effect through independent verification devices to ensure compliance with regulatory requirements such as the Biosafety Laboratory Standards.