Lactase is an enzyme that can be secreted by the gastrointestinal tract on its own, but sometimes it may be insufficient and needs to be supplemented. Lactase deficiency is divided into congenital lactase deficiency and secondary lactase deficiency; Colonic metabolic disorders, overuse of antibiotics, destruction of gut microbiota, damage to intestinal mucosa, resulting in poor sugar absorption, reduced or deficient lactase secretion in the body, thereby inducing lactose intolerance symptoms.

According to GB 2760 Food Additives Regulations, lactase is a food additive enzyme preparation that is only allowed to be used in four categories: blended milk, blended cream powder, and cream. Since it is an enzyme preparation, activity must be emphasized. Some lactase products that only have ingredient names but do not indicate activity have questionable ability to hydrolyze lactose.

The production process of lactase as a food additive is very important. So, how to produce lactase?

With the continuous breakthroughs and advances in enzyme engineering technology, membrane separation technology has been effectively applied in the production of enzyme preparations, and has produced significant effects in reducing production costs and improving product quality.

Membrane separation technology refers to the selective separation of liquid mixtures of two or more components with different particle sizes at the molecular level when passing through a specific membrane. Due to the advantages of membrane separation process, which can be carried out at room temperature without any phase or chemical changes, high separation coefficient, and continuous operation, it is particularly suitable for the separation, concentration, and purification of thermosensitive substances such as enzymes. Research has shown that enzyme preparations separated by ultrafiltration membranes have high purity and good yield.

Advantages of lactase preparation membrane separation and concentration equipment:

(1) It can be concentrated and purified at room temperature, making it easy to achieve continuous industrial production and avoiding the influence of temperature on the quality of enzyme preparations. Both solid and liquid enzymes have relatively high purity and yield.

(2) Capable of effectively concentrating low concentration enzyme solutions.

(3) Compared with vacuum evaporation, ultrafiltration technology not only has low energy consumption but also is easy to operate.

(4) Compared with traditional salt precipitation and extraction methods, membrane separation equipment can save costs and does not require the use of many inorganic salts and organic solvents.

The Literide enzyme preparation membrane separation equipment adopts membrane clarification and membrane concentration processes, which can effectively purify and concentrate enzyme preparations. Due to the low-temperature concentration of membrane concentration, the energy consumption is low and the product activity is well preserved. In addition, membrane concentration is based on the principle of molecular sieves to trap enzymes, allowing small molecule impurities and water to pass through. Therefore, during the concentration process, inorganic salts and small molecule nutrients in the fermentation broth can be effectively removed, purifying enzymes and improving the quality of enzyme preparations.