The design and construction of stem cell laboratories require comprehensive consideration of multiple factors to ensure the safety, functionality, and scientificity of the laboratory, providing a solid guarantee for stem cell research and applicationBotai editor will take you to understand relevant knowledge.

1Clearance and environmental monitoring:

Strict cleaning operation procedures should be established and a complete clean area environmental monitoring operation procedure should be established, and corresponding testing methods and frequencies should be developed for each monitoring indicator.

IIEnvironmental requirements for storage area:

The design and construction of deep low-temperature storage facilities for stem cell preparations should ensure good storage conditions, and the freezing storage temperature should not exceed-At 150 ℃, there should be ventilation and lighting facilitiesandGas monitoring facilities.

IIIDesign and layout of work area:

Each functional area should be relatively independent and meet the space, facilities, and cleanliness requirements required for its functions. The quality control area should be physically isolated from the preparation area, and administrative, living, and auxiliary areas should not hinder the preparation of stem cell preparations.

4Operation area arrangement:

Stem cell preparations with different quality standards or process specifications should be operated in different rooms.The preparation of reagents, separation, expansion, and induction of differentiation of stem cells, preparation, filling, or packaging of stem cell preparations should be carried out in separate areas within the clean zone.

5Cleanliness level requirements:

The receiving sampling work area should be isolated from the preparation area in a clean environment, and the sampling operation during receiving should be carried out at a high levelDon'tConduct in a clean environment. Cell operations that are not in a sealed state and do not come into direct contact with cells using reagents or instruments, inHigh environment under B-level background.