-
E-mail
18201882912@163.com
-
Phone
18201882912/15821268711
-
Address
No. 6848 Liuxiang Road, Huating Town, Jiading District, Shanghai
Shanghai Bailun Biotechnology Co., Ltd
Process principle and application of single arm glass fermentation tank
Date: 2025-08-20Read: 6
Single arm glass fermentation tank is a commonly used equipment in laboratories and small-scale production. Its core design integrates aseptic control, precise regulation, and efficient mixing mechanism, and is widely used in the fields of biotechnology and food industry. The following analyzes its characteristics from the perspectives of process principles and applications:
1、 Process principle
1. Aseptic environment control: The tank body is made of high borosilicate glass material, combined with sealing design and steam sterilization technology (such as 121 ℃ high-pressure sterilization), to ensure that the microbial fermentation process is free from contamination by miscellaneous bacteria; The glass surface is mirror polished (Ra ≤ 0.4 μ m) to eliminate hygiene blind spots and significantly reduce the risk of microbial adhesion.
2. Temperature precision control: By using a jacket or built-in snake tube structure to circulate temperature control media (such as hot/cold water), combined with a PID intelligent control system, an accuracy of ± 0.5 ℃ is achieved to maintain the optimal growth temperature of microorganisms (such as 25-30 ℃ for yeast).
3. Ventilation and stirring mechanism: Single arm stirring blades (such as flat blade turbine type) promote gas-liquid mixing at low speeds (200-300rpm), reducing damage to sensitive bacterial cells caused by shear forces; Simultaneously introduce sterile air (filtered through a 0.22 μ m filter) to form tiny bubbles to increase dissolved oxygen levels and optimize substance transfer.
4. Dynamic adjustment of pH and dissolved oxygen: Integrating pH electrodes and dissolved oxygen electrodes to monitor the fermentation broth status in real-time, automatically adding acidic and alkaline solutions or adjusting ventilation to maintain appropriate pH (5-7) and dissolved oxygen levels (such as DO>30% saturation), ensuring metabolic efficiency.
5. Structural optimization and energy transfer: The cylindrical tank body is designed with an elliptical head to promote uniform mixing of the culture medium; Single arm stirring simplifies the structure, reduces energy consumption, and prevents leakage through mechanical seals (such as double end seals), making it suitable for fermentation of low viscosity materials.
2、 Application Fields
1. Laboratory research and development: As a small-scale fermentation system, it is used for microbial cultivation optimization (such as bacteria and yeast), metabolite analysis, and strain screening. The glass material facilitates real-time observation of the fermentation process, making it suitable for academic research and process development.
2. Biopharmaceutical production: In the production of vaccines, antibiotics, or recombinant proteins, providing a sterile environment and precise control of parameters (such as temperature and pH), shortening the fermentation cycle, and improving product purity; For example, the single tank fermentation method simplifies the traditional two-stage process and reduces the risk of bacterial contamination.
3. Food and beverage industry: applied to yogurt, soy sauce or beer fermentation, optimizing flavor substance generation by controlling dissolved oxygen and stirring rate; In dairy products, efficient cleaning is achieved by combining CIP rotating nozzles.
