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Process principle of Escherichia coli glass fermentation tank
Date: 2025-04-27Read: 7
The process principle of Escherichia coli glass fermentation tank is based on precise control and optimization of microbial metabolic activity, and its core lies in dynamically adjusting fermentation parameters through structural design and automation system. The following is a detailed analysis of its process principle:
1、 Tank structure and material characteristics
1. Transparent glass tank body
Using high borosilicate glass material, it has both corrosion resistance and transparency, making it easy to observe the fermentation broth status and microbial growth in real time.
2. Sealing system
The two ends of the tank are sealed with stainless steel plates and rubber gaskets to ensure a sterile environment and prevent contamination by miscellaneous bacteria.
3. Multi functional interface
The tank is equipped with feeding ports, sampling valves, electrode interfaces (pH, dissolved oxygen, temperature), etc., supporting real-time monitoring and operation.
2、 Key process control module
1. Temperature control
By using electric blankets and cold water pipe modules to achieve bidirectional temperature control, combined with PID algorithm to control temperature fluctuations within ± 0.5 ℃ range.
The optimal growth temperature for Escherichia coli is 37 ℃, but the product synthesis stage may need to be adjusted to 35-36 ℃ to optimize yield (such as L-tyrosine production).
2. Dissolved oxygen (DO) management
Adopting a cascade control strategy: when the DO value is below the set threshold (such as 30%), the air and pure oxygen supply will be automatically switched, and the stirring rate will be increased to enhance the oxygen transfer efficiency.
The mixer design balances shear force and mixing efficiency, avoiding damage to bacterial cells and ensuring uniform distribution of dissolved oxygen.
3. PH dynamic adjustment
Maintain pH stability through an acid-base supplementation system to meet the needs of different growth stages of Escherichia coli (such as logarithmic growth requiring a weakly acidic environment).
3、 Feeding and metabolic regulation strategies
1. Batch feeding mode
The initial feed rate is set at 0.2 mL/min, with a growth rate of 10% to 15% every 2 hours. The specific growth rate (μ) is controlled between 0.06 and 0.08 h ⁻¹ to avoid substrate inhibition and prolong the production period.
2. Nutritional gradient control
Optimize feeding strategies by online detection of parameters such as CO ₂ release rate and substrate consumption to prevent local overnutrition or undernutrition.
4、 Process amplification and stability assurance
1. Principle of scale up
Maintain a constant ventilation volume to liquid volume ratio (VVM) (e.g. 0.2~0.3) to ensure consistent dissolved oxygen transfer efficiency.
2. Pollution prevention and control
Sterilized culture medium and aseptic inoculation technology are used in combination with defoaming device to prevent the risk of bacteria contamination caused by foam overflow.
5、 Automation and Data Integration
1. Parameter linkage control
By integrating sensors and actuators, closed-loop control of parameters such as temperature, dissolved oxygen, and pH can be achieved, reducing manual intervention errors.
2. Data traceability system
Record key parameter curves (such as OD600 and specific growth rate) to provide a basis for process optimization.
summary
The Escherichia coli glass fermentation tank achieves high-density cultivation and efficient product synthesis through the synergistic effect of structural design, dynamic parameter regulation, and automation system. Its core advantages lie in precise environmental control capabilities and scalable process scaling strategies, suitable for production needs from laboratory to pilot scale.
