The working principle of a carbon dioxide incubator is to simulate the growth environment of cells and tissues in living organisms by precisely controlling the concentration, temperature, and humidity of carbon dioxide inside the incubator. The carbon dioxide incubator mainly consists of a box body, heating system, carbon dioxide control system, humidity regulation system, air circulation system, and monitoring and alarm system. Its working principle is as follows:

• Temperature control: The temperature inside the box is maintained at around 37 ° C through a heating system, which is the temperature required for the growth of most human cells.

Carbon dioxide concentration control: The carbon dioxide control system detects and controls the concentration of carbon dioxide inside the box, usually maintaining around 5%. When the carbon dioxide concentration inside the box is detected to be low, the control system will open the solenoid valve to allow carbon dioxide to enter the box until the set concentration is reached, and then close the solenoid valve to maintain a stable state.

Humidity control: The humidity regulation system ensures that the relative humidity inside the box remains at around 95%, preventing evaporation of the culture medium and providing the best growth environment for cells.

Air circulation: The air circulation system ensures a uniform environment inside the box, avoiding excessive or insufficient local temperature and humidity.

Monitoring and alarm: The monitoring and alarm system monitors various parameters in real time, and will issue an alarm once any abnormal situation is detected.

The application fields of carbon dioxide incubators are very extensive, mainly including but not limited to:

Cell biology research: used to cultivate various cell lines, such as tumor cells, stem cells, nerve cells, etc., to study the processes of cell growth, proliferation, differentiation, and apoptosis.

• Tissue engineering and regenerative medicine: play an important role in the cultivation of tissue engineering products such as skin, blood vessels, nerves, etc., for repairing and replacing damaged tissues and organs.

Microbiological research: Cultivate various microorganisms such as bacteria, fungi, viruses, etc., and study their growth, metabolism, and pathogenic mechanisms.

Drug screening and toxicity testing: used in the pharmaceutical industry to evaluate the biological effects of drugs on cells or tissues, supporting new drug development.

Gene editing and transgenic research: providing a reliable operating environment for gene editing technology, supporting the modification and expression of genes within cells.