The operation process of the wet heat sterilization cabinet mainly includes the following key steps, which need to strictly follow the equipment specifications to ensure the effectiveness of sterilization:
1、 Equipment and item preparation
1. Equipment inspection:
Confirm that the imported steam pressure is greater than 2 bar and the pure steam pressure is greater than 1.5 bar.
Open the pure steam through valve (12) and the interlayer steam through valve (11), drain the condensed water until there is no gas escaping from the outlet, and keep the valves open.
Check the status of the power supply, compressed air valve, and pneumatic valve to ensure that there are no abnormalities.
Before loading, ensure that the items to be sterilized are clean and dry to avoid residual moisture affecting the sterilization effect.
2. Specification for loading items:
The size of the package should not exceed 300mm x 300mm x 500mm, and should not be tightly attached to the walls of the inner chamber.
The interval between packages on the same layer is ≥ 10mm, and the upper and lower layers are staggered to ensure even steam penetration.
The loading capacity shall not be less than 10% of the interior volume, and overloading is prohibited to block the airflow channel.
2、 Sterilization program execution
1. Start preheating:
Close the cabinet door and tighten it, open the interlayer steam valve (9) and the inner chamber pure steam valve (10), preheat the inner chamber for 20 minutes.
Press the start and reset buttons simultaneously for 1 minute, and discharge the pure steam inlet condensate through valve 8.
2. Parameter setting and sterilization:
Set sterilization temperature (≥ 121 ℃), sterilization time (≥ 30 minutes), vacuum pulsation frequency (≥ 4 times), and drying time (approximately 39 minutes).
3. Automatically execute after starting the program:
Vacuum pulsation: Remove the air from the inner chamber 3-4 times to enhance steam permeability.
Sterilization stage: Pure steam is injected into the inner chamber to maintain the set temperature and time, and the F0 value needs to reach the control standard (such as target value+50% safety margin).
4. Drying and Ending:
After sterilization is completed, start the vacuum pump to remove steam and dry it by heating the interlayer.
After drying, the outside air is injected into the inner chamber through a 0.22 μ m filter to balance the air pressure.
After the temperature drops to a safe range (below about 60 ℃), open the door to retrieve the item to avoid burns.
3、 Key points of safety and maintenance
1. Material limitations: Glazed tableware and wooden utensils are prohibited from using high-temperature layers, and plastic/silicone should be placed in the ozone disinfection layer.
2. Fault handling:
Abnormal temperature: Check the sensitivity of the pure steam trap or temperature probe.
Vacuum pulsation failure: Check for blockages in the power supply, water circuit, or exhaust pipe of the vacuum pump.
3. Maintenance requirements:
Power on 1-2 times a week to prevent moisture, and replace the filter regularly (every six months/year).
The device should be more than 30cm away from the wall to ensure heat dissipation, and the interior and seals should be cleaned after use.
Attention: The entire operation process requires monitoring of temperature uniformity (thermal distribution test) and loading penetration effect (thermal penetration test) to ensure sterilization consistency.