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Thermo Fisher Scientific (China) Co., Ltd
to update! Orbitrap Ascend Editions Three in One Mass Spectrometer Multiple Upgrades Collection
Date: 2025-12-19Read: 1
The Orbitrap Ascend Editions three in one mass spectrometer is a revolutionary platform that integrates multiple fragmentation and reaction technologies. It achieves data complementarity through a single system, greatly simplifying workflow and improving analysis efficiency and depth. This instrument significantly enhances its coverage in multi omics research, accelerates drug development and quality control analysis in the biopharmaceutical field, and provides powerful assistance for solving cutting-edge life science problems such as complex protein structures.
In the updated version 4.3 software, multiple upgrades have been made in the areas of data quality control, intelligent data acquisition, electron transfer lysis (ETD), oligonucleotide analysis, etc., which can significantly improve the efficiency of multi omics, biopharmaceutical, and structural biology analysis.
New: Real Time Quality Control, RT-QC
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A filtering function has been added to the method editor, Real-Time QC， Users can set an upper or lower limit for the number of identified peptide segments. During the data collection process, real-time retrieval is performed. If the results do not meet the set standards, the Xcalibur sequence will automatically pause for users to investigate possible causes and avoid wasting the remaining samples.
New: Adaptive Retention Time Adaptive RT
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The adaptive retention time function can automatically adjust the retention time of the analyte based on the collected reference spectrum file, reducing missing values and narrowing the retention time window for target quantitative analysis, effectively improving analysis throughput. Can be used for PRM quantification or SQUAD and hybridDIA methods.
In addition, the addition of AdaptiveRT can better achieve the complete process from discovery to target analysis in a single system.
New: HybridDIA
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The HybridDIA method combines non target DIA analysis and target based tMS2 analysis in the same injection, allowing for simultaneous discovery type DIA and target quantification. It is suitable for focusing on specific target objects while non target analysis.
In the Ascend instrument, parallel analysis can be better achieved by using both Orbitrap and ion trap mass analyzer. Orbitrap is used for DIA scanning and ion trap is used for targeted MS2 scanning, achieving high sensitivity and high-throughput quantification of specific targets without sacrificing DIA depth coverage performance.
Update: Real time retrieval of RTS
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The RTS SPS MS3 method can significantly improve quantitative accuracy in TMT experiments, and the newly added signal-to-noise ratio threshold filtering can further enhance quantitative credibility.
When real-time retrieval (RTS) is enabled and the Closeout function is enabled in TMT SPS MS3 mode, the interface will display the newly added 'MS3 signal-to-noise ratio threshold' option, which can be customized. If the MS2 spectrum identified through real-time search generates a low-quality MS3 spectrum, it will no longer be included in the quantitative values of the corresponding proteins, which is more helpful in improving the accuracy of quantification.
Update: Real time spectral library retrieval RTLS
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Real time library search (RTLS) can achieve intelligent collection of similar compounds in the database. In the small molecule analysis mode, the newly added Modified Cosine Similarity takes into account the mass deviation between parent ions when comparing experimental spectra and library spectra, and corrects the mass to charge ratio of fragment ions accordingly, thereby calculating similarity more accurately.
Using the updated RTLS, no manual intervention is required, and structurally related compounds can be automatically discovered through library searches. And due to higher matching scores, more matching compounds can be discovered in unknown samples.
Update: Faster ETD response time
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ETD fragmentation has important applications in various research fields, including glycosylation modification, disulfide bonding, de novo sequencing, top-down, middle down analysis, and so on. Among them, ETD reaction time is a very important parameter that affects fragmentation efficiency, overall cycle time, and so on.
After the update, the ETD reaction time in the ion trap is shortened, closer to the ion implantation time, which can achieve better parallelization efficiency.
New: HDF calibration settings
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In hydrogen deuterium exchange mass spectrometry analysis, the ETD method can significantly improve resolution and be accurate from the peptide level to the level of individual amino acid residues, which is particularly important for applications such as epitope analysis that require precise localization of binding sites.
Among them, minimizing scraping is crucial. The newly added HDF calibration function can automatically adjust instrument parameters with one click settings, achieving milder ion transport, reducing hydrogen deuterium scrambling in HDF-ETD experiments, and obtaining more accurate positioning of interaction sites.
New: Oligonucleotide mode
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The current oligonucleotide analysis faces multiple challenges:
●High spectral complexity
●There are a large number of homogenous and heterogeneous fragment ions present
●Generate internal fragments that are difficult to parse
In the newly added oligonucleotide mode, using low Mathieu q-value CID technology and correcting it can effectively reduce the complexity of the secondary spectrum and improve the sequence coverage of oligonucleotide analysis. Users can operate by calling the oligonucleotide characterization method template, which is simple and easy to use.
The multiple upgrades of the Orbitrap Ascend Editions three in one mass spectrometer have successfully pushed the coverage of multi omics research, the robustness of biopharmaceutical development, and the analytical accuracy of structural biology to new heights by integrating smarter real-time acquisition control, faster ETD reactions, and solutions designed specifically for complex characterization of protein advanced structures, oligonucleotides, and more.