Bioengineering fermentation tanks are key equipment used in the field of biotechnology for microbial or cell culture, providing a suitable environment to promote the growth of organisms and the generation of metabolic products. The design and use of fermentation tanks are directly related to the quality and yield of biotechnology products.
Bioengineering fermentation tankStructural design:
1. Tank: A container that holds culture medium, usually designed in a cylindrical shape for easy mixing and heat transfer.
2. Mixing system: including mixer, mixing shaft, and driving motor. The agitator is located at the bottom of the tank and is connected to the motor through the agitator shaft to maintain uniform distribution of cells or microorganisms in the culture medium and promote the transfer of oxygen and other nutrients.
3. Temperature control system: A heating/cooling system in the form of a jacket or coil is used to maintain a constant temperature inside the tank.
4. Ventilation system: sterile air is delivered into the tank through an air filter to ensure the cultivation needs of aerobic microorganisms.
5. PH and dissolved oxygen monitoring system: Real time monitoring and control of the pH value and dissolved oxygen level of the culture medium to ensure a suitable growth environment.
6. Feeding and discharging port: used to supplement nutrients or discharge culture medium during the cultivation process.
7. Sampling port: used to take out samples without opening the tank to monitor the cultivation status.
Operating principle:
1. Sterilization: Before cultivation, sterilize the fermentation tank and its ancillary equipment with high-temperature steam to ensure sterile conditions.
2. Inoculation: Transfer pre cultured microorganisms or cell seeds into a fermentation tank.
3. Cultivation: Under constant temperature, pH value, and oxygen supply, the uniformity of the culture medium is maintained by stirring to promote the growth of organisms.
4. Monitoring: Real time monitoring of temperature, pH value, dissolved oxygen and other parameters of the culture medium through sensors, and adjusting as needed.
5. Harvest: At the end of the cultivation cycle, collect the culture medium and separate the target product through centrifugation, filtration, and other methods.
Application areas of bioengineered fermentation tanks:
1. Medicine: Production of antibiotics, vaccines, enzymes, and other bioactive substances.
2. Food: Production of yeast, lactic acid bacteria, vitamins and other food additives.
3. Environmental protection: Treat wastewater and exhaust gas, and degrade harmful substances through microorganisms.
4. Chemical industry: producing renewable resources such as biofuels and bioplastics.