Chinese Pharmacopoeia 2025 Edition - Determination of Ciwujia Extract Content

This product is a processed extract of Ciwujia. [Method] Take Ciwujia1000gCrush into coarse powder, boil twice with water, each time3After hours, combine the decoction, filter, and concentrate the filtrate into a paste50g(Water extract), obtained immediately; Or take the Thorn Five Plus1000gCrush into coarse powder and add75%Ethanol, reflux extraction12Hour, filter, recover ethanol from the filtrate until there is no alcohol odor, and concentrate into a paste40g(Alcohol extract), ready to use.

【 Appearance 】 This product is a thick, dark brown paste like substance; Fragrant aroma, slightly bitter and astringent taste.

使用 Positisil ODS-AQ 120A 5um 4.6X250mmThe chromatographic column was used to determine the content of Ciwujia extract by high performance liquid chromatography according to the 2025 edition of the Chinese Pharmacopoeia. The detection of syringin (C17H24O9), Ciwujia glycoside E (C34H46O18), and isoquinoline (C11H10O5) met the experimental requirements.

1. Chromatographic conditions and system suitability test:

chromatographic columnPositive ODS-AQ 120A 5um 4.6X250mmm

Mobile phaseUsing acetonitrile as mobile phase A and 0.1% phosphoric acid solution as mobile phase B, perform gradient elution as specified in the table below;