QDrop-100 Digital PCR System

1、 QDrop-100 Digital PCR SystemTechnical Principle

Digital PCR (dPCR) technology is currently an advanced third-generation absolute quantification technique for nucleic acid molecules. The basic principle is to uniformly disperse the PCR reaction system into tens of thousands of independent reaction units, and after amplification, perform Poisson distribution statistical analysis on the fluorescence signals of each reaction unit. Compared to gPCR, digital PCR can directly count the number of DNA molecules and has the characteristics of high sensitivity and absolute quantification.

2、 System Introduction

QDrop-100 Digital PCR SystemIncluding droplet generators and droplet readers. Adopting the core technology of droplet generation with independent intellectual property rights, we have innovatively developed droplet generation chips and digital PCR systems, which are characterized by simple operation, high sensitivity, and high throughput.

3、 Product Features

Easy to operate: No droplet transfer manual operation

High throughput: compatible with 96 well plates

Low cost: lower instrument and consumable costs

4、 Operation process

5、 System performance

6、 Technical parameters

1. Sample size: 20uL

2. Droplet generation flux: 1-8 samples/droplet generation chip

3. Total number of droplets/sample: 20000

4. Droplet generation time:<3 minutes/time

5. Reading instrument flux: 1-96 samples

6. Detection method: Flow droplet detection

7. Excitation light source: solid-state dual laser

8. Detector: Silicon Photon Counter

9. Detection channels: FAM (EvaGreen), HEX (VIC)

10. Accuracy: ± 10%

11. Linear range: 5 orders of magnitude

12. Testing time:<3 minutes/sample