Rhubarb chromatographic column

Chinese Pharmacopoeia 2025 Edition - Determination of Rheum Content

This product is the dried roots and rhizomes of the Polygonaceae plants Rheum palmatum L., Rheum tanguticum Maxim. ex Balf., or medicinal Rheum officinale Baill. At the end of autumn, when the stems and leaves wither or sprout in the following spring, dig them up, remove the fine roots, scrape off the outer skin, cut the petals or segments, and string them together to dry or directly dry.

【 Functions and Indications 】 Diarrhea and attack accumulation, clearing heat and fire, cooling blood and detoxifying, removing blood stasis and promoting menstruation, promoting diuresis and reducing jaundice. Used for constipation caused by excessive heat accumulation, blood heat vomiting and bleeding, redness of the eyes and throat swelling, carbuncles and sores, abdominal pain caused by intestinal abscess, obstruction of blood stasis meridian, postpartum blood stasis, traumatic injury, damp heat dysentery, jaundice and red urine, gonorrhea, edema; External treatment for burns and scalds. Jiu Da Huang is good at clearing the heat and toxins in the upper burner blood, and is used for treating redness and swelling of the eyes, throat, and gums. Ripe rhubarb has a slow laxative effect, detoxifies fire, and is used for treating fire ulcers. Dahuang charcoal is used to cool blood, remove blood stasis and stop bleeding, and is used for the treatment of blood heat and blood stasis.

Using Positsil ODS-2 120A 5um 4.6X250mmThe chromatographic column was used to determine the content of rhubarb using high-performance liquid chromatography according to the 2025 edition of the Chinese Pharmacopoeia. The total anthraquinone and free anthraquinone were detected, including aloe emodin (C15H10O5), rhein (C15H8O6), emodin (C15H10O5), emodin (C15H10O4), and emodin methyl ether (C16H12O5). The results obtained meet the experimental requirements.