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Shenzhen Kemis Technology Co., Ltd
YMC BioPro ion exchange filler
NegotiableUpdate on 03/04
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Overview
YMC BioPro ion exchange filler adopts a hydrophilic porous polymer matrix, which has the characteristics of low non-specific adsorption and high adsorption capacity. It is very effective for the initial purification (Capture: high exchange capacity) to intermediate purification (Intermediate: high efficiency) of biomolecules. YMC can provide various products such as anion exchange Q, cation exchange S, weak anion exchange DA, and weak cation exchange CM.
Product Details
YMC BioPro Q-type anion exchange filler
YMC-BioPro Q Anion Exchange Media
YMC-BioProThe ion exchange filler adopts a hydrophilic porous polymer matrix, which has the characteristics of low non-specific adsorption and high adsorption capacity, and is suitable for the initial purification of biomolecules（CaptureFrom high exchange capacity to intermediate purification（IntermediateBoth are highly effective, and YMC can provide anion exchangeQCation exchangeSWeak anion exchangeDAAnd weak cation exchangeCMWaiting for multiple products.
Technical Parameter
| substrate | Particle size | functional group | PH range | ||
|---|---|---|---|---|---|
| BioPro Q | Hydrophilic porous polymer | 75 μm | -R+(CH3)3 | 2.0-12.0 | |
| BioPro S | 75 μm | -R-SO3- | |||
| BioPro DA | 60 μm | -R-N(CH3)2 |
Long term: 3.0-12.0 Transient: 1.0-13.0 |
||
| BioPro CM | 60 μm | -R-COOH | |||
Characteristic 1: High Dynamic Adsorption Capacity (DBC)
BioPro ion exchange fillers have a higher dynamic adsorption capacity (DBC) than most commercially available products, making them particularly suitable for capture steps in protein purification.
| Anion exchange filler | Particle size (μ m) | Ion exchange capacity (meq/mL resin) | DBC * (mg/mL resin) |
|---|---|---|---|
| BioPro Q75 | 75 | 0.13 | 183 |
| Brand G (porous Q type) | 90 | 0.19 | 102 |
| Cation exchange filler | Particle size (μ m) | Ion exchange capacity (meq/mL resin) | DBC * (mg/mL resin) |
|---|---|---|---|
| BioPro S75 | 75 | 0.12 | 192 |
| Brand G (porous S type) | 90 | 0.13 | 80 |
*DBC is determined at 10% breakthroughunder following conditions:
| chromatographic column | ： | 50 X 4.6 mmI.D. |
|---|---|---|
| Linear flow velocity | ： | 3.0 cm/min (180 cm/hr) |
Anion exchange filler
| Equilibrium solution | ： | 20 mM Tris-HCl (pH 8.6) |
|---|---|---|
| Elution solution | ： | 0.5 M NaCl in equilibration buffer |
| sample | ： | 1.5 mg/mL BSA in equilibration buffer |
| detected condition | ： | UV at 280 nm |
Cation exchange filler
| Equilibrium solution | ： | 20 mM Glycine-NaOH (pH 9.0) |
|---|---|---|
| Elution solution | ： | 0.5 M NaCl in equilibration buffer |
| sample | ： | 1.5 mg/mL Lysozyme in equilibration buffer |
| detected condition | ： | UV at 300 nm |
Feature 2: Maintain high DBC even at high flow rates
When the flow rate increases from 200cm/hr to 1000cm/hr, the change in DBC is less than 5%, indicating that BioPro ion exchange packing can maintain high dynamic adsorption capacity (DBC) over a wide range of flow rates. BioPro ion exchange fillers can effectively increase yield and reduce production costs in biopharmaceutical production.
| chromatographic column | ： | 50 X 5.0 mmI.D. |
|---|---|---|
| Equilibrium solution | ： | 20 mM Glycine-NaOH (pH 9.0) |
| Elution solution | ： | 0.5 M NaCl in equilibration buffer |
| sample | ： | 1.0 mg/mL Lysozyme in equilibration buffer |
| detector | ： | UV at 300 nm |
Characteristic 3: Excellent Alkali Resistance (CIP)
For protein purification, it is necessary to clean and sterilize the column with alkaline solution (CIP). BioPro ion exchange packing, due to its excellent chemical stability, can effectively withstand 1M NaOH alkaline cleaning, thus maintaining high DBC and selectivity even after 20 CIP implementations.
DBC testing conditions
| chromatographic column | ： | BioPro S75 50 X 5.0 mmI.D. |
|---|---|---|
| velocity of flow | ： | 800 cm/hr |
| Equilibrium solution | ： | 20 mM Glycine-NaOH (pH 9.0) |
| Elution solution | ： | 0.5 M NaCl in equilibration buffer |
| sample | ： | 1.0 mg/mL Lysozyme in equilibration buffer |
| detected condition | ： | UV at 300 nm |
※DBC is determined at 10% breakthrough
production list
| Packing name | Particle size (μ m) | Item number P/N |
|---|---|---|
| BioPro Q75 (Anion) | 75 | QAA0S75 |
| BioPro S75 (cationic) | 75 | SPA0S75 |
| BioPro DA60 (weak anion) | 60 | DAM99S60 |
| BioPro CM60 (weakly cationic) | 60 | CMM99S60 |
Application example:Purification of IgY from Egg Yolk Using YMC BioPro Q75 Ion Exchange Filler and YMC Diol Volume Exclusion
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