/Reagent information/

CAS number: 459426-22-3

Chinese name: Biotin polyethylene glycol 4-N-hydroxysuccinimide ester; Bio-PEG4 active ester

English name: Biotin-PEG4-NHS; NHS-PEG4-Biotin ； Biotin-Polyethylene Glycol4-N-Hydroxysuccinimide

Appearance: White to off white solid or powder

Solubility: soluble in water, DMSO, DMF, chloroform, etc

Purity: 95%+

Transportation: Low temperature transportation

Application: Biomolecular markers

Supplier: Xi'an Kaixin Biotechnology Co., Ltd

Purity: ≥ 95%

Structural formula:

Security protection:

During the operation, disposable protective clothing, masks and other protective equipment should be worn to ensure self safety.

This reagentonly useIn scientific research

Attention: When using, be sure to dry and avoid frequent dissolution and freeze-drying

/Reagent description/

Biotin-PEG4-NHS, as a key bio coupling reagent, consists of three parts: biotin, tetrapolyethylene glycol (PEG4), and NHS ester.

Biotin has the characteristic of specific binding with avidin or streptavidin. Based on this high affinity interaction, labeled biomolecules can be easily detected or separated.

PEG4 chains exhibit excellent hydrophilicity and flexibility, which can enhance the water solubility of molecules, reduce non-specific adsorption, and also play a spacer role to ensure the normal functioning of biotin and NHS esters.

NHS esters belong to active groups and can react with amino groups under mild reaction conditions to form stable amide bonds, thereby achieving coupling with amino containing biomolecules or materials.

/Overview of synthetic routes/

The synthesis of Biotin-PEG4-NHS generally adopts a segmented connection strategy:

PEG4‑NHS 前体

The PEG4-NHS intermediate was obtained by esterification of tetrapolyethylene glycol diol (HO - (CH ₂ CH ₂ O) ₄ - OH) with N-hydroxysuccinimide (NHS) under nitrogen protection.

Biotin grafting

The carboxyl group (or activated carboxylic acid) of biotin is amidated with PEG4-NHS through DCC/DMAP or EDC/NHS systems to obtain Biotin-PEG4-NHS.

purification

Unreacted NHS, PEG4-NHS, and by-products were removed by silica gel column chromatography (using DCM/MeOH gradient) or reverse phase HPLC, resulting in a product with a purity of ≥ 95%.

This process is carried out under anhydrous and inert atmosphere (nitrogen or argon) to prevent hydrolysis of NHS esters.

/Related reagents/

mPEG-I / mPEG-I

PLGA-SH PLGA-SH

mPEG-HZ / mPEG-HZ

mPEG-VD / mPEG-VD

mPEG-PLGA-PNIPAm

mPEG-Asp / mPEG-Asp

mPEG-PFP / mPEG-PFP

mPEG-DOPE / mPEG-DOPE

mPEG-Pyrene / mPEG-Pyrene

mPEG-Dansyl / mPEG-Dansyl

NHS-PEG-Mal / NHS-PEG-Mal

NHS-PEG-NHS / NHS-PEG-NHS

mPEG-Dextran / mPEG-Dextran

mPEG-Vitamin E / mPEG-Vitamin E

PLGA-PEG-Acrylate,Acrylate-PEG-PLGA

mPEG-Glutamic Acid / mPEG-Glutamic Acid

The above content is edited and organized by YNN of Xi'an Kaixin Biotechnology Co., Ltd

This reagent is only used for scientific research experiments and cannot be used on the human body