·Chemical composition and structure

Biotin PEG NH ₂ is a bifunctional molecule composed of three main parts connected by covalent bonds:

Biotin: a vitamin (B7) that can form specific non covalent bonds with avidin or streptavidin.

Polyethylene glycol (PEG) chain: As a flexible connecting arm, its length (molecular weight) can be adjusted, commonly available in 1k, 2k, 3.4k, 5k, 10k and other specifications. PEG chains endow molecules with good water solubility, biocompatibility, and can reduce non-specific adsorption.

Terminal amino group (- NH ₂): provides the main chemical reaction site and can undergo coupling reactions with carboxyl, aldehyde, active ester and other functional groups to achieve connection with the target molecule

·Basic performance parameters

Chinese name: Biotin PEG amine; Biotin Polyethylene Glycol Amino

English name: Biotin-PEG-NH2; Biotin-PEG-amine

Purity: 95%+

Transportation: Low temperature transportation

Molecular weight: 1k, 2k, 3.4k, 5k, 10k, 20k, etc

Purity: ≥ 95%

Structural formula:

Attention: When using, be sure to dry and avoid frequent dissolution and freeze-drying

Appearance: Solid powder or granules

Solubility: soluble in some organic solvents and water

Storage conditions: Freeze and dry below -20 ℃ to avoid repeated freeze-thaw cycles

Packaging specifications: 1g, 5g, 10g

Security protection:

During the operation, disposable protective clothing, masks and other protective equipment should be worn to ensure self safety.

This reagentonly useIn scientific research

·Synthesis methods and process optimization

Amide bond formation method

Step 1: Biotin COOH reacts with NHS (N-hydroxysuccinimide) in dry DMF to form the active ester Biotin NHS.

Step 2: Biotin NHS reacts with amino polyethylene glycol (HO-PEG-NH ₂) in PBS (pH 7.4) to form Biotin PEG NH ₂.

Advantages: Mild reaction conditions, high yield, suitable for large-scale synthesis.

Direct modification method

If the end of PEG is carboxyl (- COOH), it can react with azidoacetic acid (N ∝ - CH ₂ - COOH) under EDC/NHS catalysis to generate azide modified Biotin-PEG-N ∝, which can be used for click chemistry or subsequent functionalization.

If the end of PEG is bromo (- Br) or iodo (- I), it can react with NaN3 in DMF and generate an azide group through nucleophilic substitution.

Purification and Quality Control

After synthesis, unreacted raw materials and small molecule by-products need to be removed by dialysis (molecular weight cut-off: 1 kDa) or high performance liquid chromatography (HPLC) to ensure product purity (usually ≥ 95%).

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The above content is edited and organized by YNN of Xi'an Kaixin Biotechnology Co., Ltd

This reagent is only used for scientific research experiments and cannot be used on the human body