Experimental ultrafiltration system LDS-L2
Tangential flow filtration

Tangential Flow Filtration (TFF) is currently a widely used membrane separation method. The sample is driven by pressure, and the contents are separated by membrane filtration according to different molecular sizes, achieving the goals of removing pyrogen, displacing solution environment, purification, and concentration.

The membrane components for tangential flow filtration are usually membrane packs or hollow fiber columns, both of which have multiple models to meet different usage needs. The type and pore size of the membrane material, solution circulation flow rate, pressure on both sides of the membrane, sample temperature, process time, and other parameters are very important for tangential flow filtration. They determine the final process effect, as well as the feasibility and specific plan for large-scale production. The tangential flow filtration equipment at the laboratory scale is dedicated to obtaining feasible process solutions and reliable process parameters, determining suitable process parameters for large-scale experiments and production, and providing effective basis for equipment or system selection.

Liposomes encapsulating small molecule drugs and lipid nanoparticles encapsulating nucleic acid drugs are currently two very popular types of drug carriers, which require tangential flow filtration for sample processing in both research and production.

Figure 1: Flow Chart of Ultrafiltration System

According to the retention pore size of the filter membrane, filtration can be classified into clarification filtration, microfiltration, ultrafiltration, nanofiltration, and reverse osmosis. Filtering membranes with different pore sizes using tangential flow operation can be referred to as tangential flow microfiltration and tangential flow nanofiltration.

Figure 2: Classification of filter membrane pore size

EitzenExperimental ultrafiltration system LDS-L2function

This system is mainly applied in the fields of sample concentration, purification, dialysis, replacement buffer, and solution heat removal. During the process, the weight of the sample, pre membrane pressure, reflux pressure, permeation pressure, transmembrane pressure TMP, and other data can be displayed and recorded through a paperless recorder or LCD panel.

The application areas include:

Ÿ Purification of chemical drug liposomes, such as doxorubicin hydrochloride liposomes, irinotecan liposomes, etc;

Ÿ Purification of nucleic acid drugs and vaccines, such as lipid nanoparticles, polymer nanoparticles, mRNA LNP, siRNA LNP, etc;

Ÿ Separation and purification of biological cell products, which can collect cells from fermentation broth and remove cell debris, etc;

Ÿ Purification of vaccines, monoclonal antibodies, and recombinant protein drugs;

Ÿ Displacement buffer solution;

Ÿ Other applications for separation and purification based on the principle of molecular size.

Technical Specifications

Aitson can customize design and integration according to user needs, and its technical parameters will be provided separately. Customizable types include:

Ÿ Different sample volumes;

Ÿ Requirements for different control methods;

Ÿ Requirements for different levels of configuration;

Ÿ General/specific type requirements;