DECA XP Plus American Thermoelectric Liquid Mass Spectrometry Instrument

Thermo Finnigan LCQ DECA XP Plus liquid chromatography-mass spectrometry is a multifunctional analysis platform based on ion trap technology, and its core performance is reflected inHigh sensitivity multi-stage mass spectrometry capability、Wide dynamic range detectionandAdaptability of complex matrix analysisThree major areas. The following is a detailed performance analysis of the system:

1、 Core technical parameters and hardware configuration

1. Quality Analysis System

• Ion trap technologyAdopting a three-dimensional quadrupole ion trap design, with a range of mass to charge ratiosm/z50-4000 (protein detection limit up to 60000 Da), supporting up to 10 levels of tandem mass spectrometry (MS)^n，n=10）The signal-to-noise ratio is 50:1.

• Sensitivity and accuracyThe detection limit is as low as 10 pg, with a mass accuracy of<0.1 Da, suitable for trace substance analysis.

2. Ion source and liquid phase system

• Electrospray ionization source (ESI): The spray voltage is adjustable from 0-8000 V, the capillary temperature is 135-350 ℃, and it is suitable for the analysis of thermally unstable compounds.

• Liquid chromatography combinedStandard binary pump system (pressure resistance of 5000 psi), flow rate of 0.01-10 mL/min, compatible with sub-2 µ m chromatographic columns, and DAD detector wavelength range of 190-950 nm.

3. Injection and Temperature Control

• Dual mode injectionSupports automatic sampler (120 bit sample tray) and flow injection injection, with sheath gas/auxiliary gas flow rates of 100 arb and 20 arb, respectively.

• Column temperature control boxThe temperature control range is 4-40 ℃ to ensure the separation stability of complex samples.

2、 Intelligent functions and workflow

1. Data Collection and Analysis

• Multi mode scanningFull scan, selective ion monitoring (SIM), data dependent scanning (DDA), etc., combined with Xcalibur software to achieve automated data processing.

• Structural analysis abilityThrough multi-level mass spectrometry fragment comparison, it supports the identification of unknown compound structures, especially suitable for natural product and metabolite research.

2. Method development efficiency

• Quick switching functionComplete ESI/APCI ion source switching within 5 minutes to meet the analysis requirements of different polar compounds.

• Dynamic range optimizationThe linear dynamic range reaches 5 orders of magnitude, meeting the quantitative requirements from trace to high concentration samples.

3、 Application scenarios and performance validation

1. Drug development and metabolic research

• Analysis of drug metabolites in blood/urine: detection limit up to10^{-16}mol， Support pharmacokinetic research.

2. Environment and Food Safety

• Pesticide residue detection: The detection limit for polycyclic aromatic hydrocarbons under the EU SANTE standard is 0.25 pg.

3. proteomics

• Peptide sequence analysis: Combining Bioworks software to achieve database matching and identify low abundance proteins.

4、 System reliability and scalability

• modular designCan be upgraded to Orbitrap high-resolution modules or combined with UPLC systems to improve resolution and throughput.

• Maintain convenienceThe ion source component supports quick disassembly with an annual downtime rate of less than 5%.

This platform relies onLevel 10 MS^nIn-depth analysisandPG level sensitivityBecoming an ideal tool for identifying small molecule structures and analyzing complex matrices.