Agilent BioTek Synergy SeriesMulti functional enzyme-linked immunosorbent assay (ELISA) reader

Agilent BioTek Synergy SeriesMulti functional enzyme-linked immunosorbent assay (ELISA) readerintroduction

The Agilent BioTek Synergy series is a modular and multifunctional microplate detection platform, including multiple models such as H1, H4, LX, and SLF. It integrates multiple detection modes including light absorption (Abs), fluorescence intensity (FI), chemiluminescence (Luminescence), time-resolved fluorescence (TRF), fluorescence polarization (FP), AlphaScreen/AlphaLISA, etc. With top-notch optical technology, flexible system configuration, and powerful automation capabilities, this series has become a core tool in drug development, life science research, and clinical diagnosis.

Core technology innovation and performance advantages

Modular optical system design:

The Synergy series adopts Hybrid Technology ™ Hybrid technology combines the flexibility of gratings with the high sensitivity of filters. The model (such as LX) is equipped with a four grating monochromator system (dual gratings for excitation and emission paths), with adjustable bandwidth (2.5-50 nm). Users can optimize detection parameters according to experimental needs, achieving the best balance between sensitivity and specificity. Its fluorescence detection sensitivity reaches 0.25 fmol fluorescein (384 well plate), and its chemiluminescence detection sensitivity reaches 10 amol ATP (flash mode).

Full wavelength coverage and advanced detection capabilities:

The wavelength range is extended to 200-999 nm, supporting nucleic acid detection in the ultraviolet region and protein analysis in the visible light region. Equipped with dual PMT detectors, it can synchronously collect chemiluminescence and fluorescence signals, greatly improving detection flux. Specially optimized for applications such as TRF, FP, and BRET, effectively eliminating background interference, suitable for kinase activity determination, molecular interaction research, and GPCR signal transduction analysis.

Precision environmental control and dynamic monitoring:

Integrated precise temperature control system (4 ° C to 65 ° C), CO ₂/O ₂ gas control module (optional), and multi-mode oscillation function, supporting long-term experiments on live cells and enzyme kinetics research. The dual syringe automatic injection system can trigger millisecond level rapid reactions and is suitable for real-time kinetic analysis such as calcium flow detection.

Intelligent automation and high-throughput integration

The Synergy series supports 6-1536 well plate detection and can seamlessly integrate with BioStack microplate stackers or robotic arms to achieve unmanned high-throughput screening (HTS). Its compact design (such as H1) provides performance comparable to large systems while saving space, while LX and other models have automatic filter switching and Z-height adjustment functions, suitable for various types of microplates and 3D cell sphere detection.

Widely applicable scenarios

The application of the Synergy series covers the multidimensional needs of modern biological research:

Drug screening: high-throughput compound activity screening, cytotoxicity evaluation, target binding affinity determination (FP/TRF)

Cell Biology: Cell proliferation/apoptosis detection, reporter gene analysis (dual luciferase), reactive oxygen species and calcium flow monitoring

Immunology and Molecular Interactions: ELISA, Protein Quantification, Kinase Activity Detection, Receptor Ligand Binding Studies

Nucleic acid testing: analysis of nucleic acid concentration and purity (A260/A280), validation of qPCR results

Gen5 software and compliance support

The powerful Gen5 microplate data acquisition and analysis software provides an intuitive graphical interface and pre-set application templates, supporting the full process management from experimental design to advanced data analysis (such as curve fitting, IC50/EC50 calculation). The software complies with the 21 CFR Part 11 compliance requirements, has complete audit tracking capabilities, and meets the data integrity requirements in GMP/GLP environments.

summary

The Agilent BioTek Synergy series representsMulti functional enzyme-linked immunosorbent assay (ELISA) readerTechnology has achieved the unity of detection performance and flexibility through modular design. Its optical system, precise environmental control, extensive automation compatibility, and compliance support enable it to tackle various challenges ranging from basic research to large-scale drug discovery. Whether used for target validation, lead compound optimization, or cell analysis, the Synergy series provides reliable data quality and operational efficiency, making it an important strategic platform for modern integrated laboratories.