Triple series quadrupole liquid chromatography-mass spectrometer

Agilent 6490Triple series quadrupole liquid chromatography-mass spectrometerLC/MS/MS is a fourth generation high-performance mass spectrometry system, whose core innovation lies in iFunnel technology, which achieves sensitivity order of magnitude improvement through three major technological breakthroughs:

1. Triple series quadrupole liquid chromatography-mass spectrometerIon source and transport system
   By using a six hole parallel sampling capillary array, the ion capture efficiency is 6 times higher than that of a single hole design. Combined with a two-stage ion funnel (high-pressure removal of neutral gases, low-pressure ion focusing) and Agilent jet focusing technology (high-temperature sheath gas assisted desolvation), the ion sensitivity below 900 m/z is increased by 10 times, and the average improvement over the entire mass range is 5-10 times. Negative ion mode optimization is more significant, with a detection limit of up to zettomole（10−2110^{-21}10−21At the mol level, a sample volume of 200 zeptomole on the column can still stably detect verapamil.

2. Triple series quadrupole liquid chromatography-mass spectrometerMass spectrometry analysis performance
   Quality range 10-2000 amu, scanning speed 10000 Da/s, dynamic range6×1066×10^66×106Supports single analysis of 40000 MRM channels. The 90 ° curved cone collision cell combined with high-pressure linear acceleration technology reduces noise and eliminates memory effects, with a RSD of less than 6% for 3000 consecutive injections (20 fg of verapamil plasma sample). The dual pole detector enhances the sensitivity of negative ions, with a mass axis drift of<± 0.1 Da/48 hours.

3. Chromatographic Compatibility and Applications
   Used in conjunction with 1290 Infinity UHPLC, microfluidic technology enables zero dead volume connection and retention time locked (RTL) deviation<0.1%. Suitable for:

• Pharmaceutical/ClinicalThe quantification limit of plasma drugs (such as fluticasone) is 5 pg/mL (2.5 fg column loading volume), and solid-phase extraction is not required;

• food safetyBuilt in MRM database for 600 pesticides and 130 toxins;

• proteomicsOn a 2 mm chromatographic column, the linear range of the 28 amol peptide segment (IEDIVTSEK) is 28-2830 amol（R2=0.997R^2=0.997R2=0.997）The detection limit is 1 amol.

4. Intelligence and Maintenance
   MassHunter software supports automatic parameter optimization (small molecules/peptides), and EMF system monitors component status in real-time. 30 ms polarity switching speed, reach working vacuum (255 L/s turbo pump) in 3 minutes, and quickly replace ion source in 5 minutes.

This instrument redefines the sensitivity boundary of ultra trace analysis through iFunnel technology, while balancing high-throughput (>10000 samples/day) and stability, making it a device for environmental monitoring, precision medicine, and other fields.