Q-TOF quadrupole time-of-flight liquid chromatography-mass spectrometry instrument

The Waters Micromass Q-TOF quadrupole time-of-flight liquid chromatography-mass spectrometer is a hybrid mass spectrometry platform that combines quadrupole mass filtration with high-resolution time-of-flight analysis. Its core performance is reflected inultra-high resolution、Multi dimensional separation capabilityandIntelligent workflowThree major areas. The following is a detailed performance analysis of the system:

1、 Core technical parameters and hardware configuration

1. Quality Analysis System

• Hybrid Quadrupole TOF ArchitectureQuadrupole rod (MS1) achieves ion pre screening, provided by orthogonal time-of-flight analyzer (MS2)m/zThe ultra wide detection range of 20000 is 100 times more sensitive than tandem quadrupole mass spectrometry.

• Resolution and accuracyV mode resolution of 10000 FWHM (W mode 17500 FWHM), mass accuracy<5 ppm (@ 1000 Da), supports precise identification of multi charged ions.

2. Ion source and separation technology

• Multi mode ion sourceCompatible with ESI, APCI, APPI, and Direct Analysis Techniques (DESI/DART), with quick switching within 5 minutes.

• T-Wave ion mobilityAdd collision cross section (CCS) separation dimension to distinguish between isomers and improve selectivity by 30%.

3. Liquid coupling system

• UPLC compatibilityCombined with Waters 2695 HPLC, using a quaternary gradient pump with a flow rate of 0.01-10 mL/min, a pressure resistance of 5000 psi, and compatible with a sub 2 µ m chromatographic column.

2、 Intelligent functions and innovative technologies

1. Data Collection and Analysis

• HDI MALDI imagingCombining PCA/OPLS-DA statistical tools to visualize the spatial distribution of compounds in tissue slices.

• FractionalLynx softwareSupport UV/MS multi signal triggered fraction collection, 24-hour unmanned operation.

2. Method development efficiency

• QuanTOF technologyThe dynamic range reaches 5 orders of magnitude, and the Fast DDA mode increases non targeted screening throughput by 50%.

3、 Application scenarios and performance validation

1. Biomedical field

• Proteomics: Identify low abundance proteins (such as horse heart myoglobin, 166 ion count) within the calibration range of 16000 Da.

2. Environment and Food Safety

• Pesticide screening: The detection limit under the EU SANTE standard is 0.25 pg (such as dioxins).

3. Materials Science

• Polymer analysis:m/zAnalyze high molecular weight distribution in 100000 mode.

4、 System reliability and scalability

• modular designUpgradeable to SYNAPT G2 Si platform, integrating DESI imaging or metabolomics analysis.

• Maintain convenienceRefactor TOF design ensures long-term stability with an annual downtime rate of less than 5%.

summary

This platform relies on20000 Da ultra wide mass range、CCS auxiliary structure analysisandMulti modal imaging capabilityBecoming a device for complex sample analysis, especially suitable for drug development and omics research