Triple quadrupole liquid chromatography-mass spectrometer

Agilent 6400 seriesTriple quadrupole liquid chromatography-mass spectrometerLC-MS/MS is a flagship platform designed specifically for high-sensitivity quantitative analysis, and its core performance is reflected inUltra high sensitivity and high-throughput analysisandIntelligent workflowThree major fields, suitable for harsh application scenarios such as pharmaceuticals, food safety, and environmental monitoring.

1、 Core technical parameters and hardware innovation

1. ion source system

• Multi mode compatibilityStandard ESI/APCI source, optional AJS (Agilent jet), APPI, and HPLC Chip nanoflow technology. The ion source temperature range is up to 300 ° C and supports flow rates from 1 µ L/min to 3 mL/min, significantly improving the ionization efficiency of trace compounds.

• IFunnel ion funnel technology(Model 6495): Enhanced ion transport efficiency through a six pole design, achieving detection limits from Ak level (10 ^ -18 g) to Zeptomole level (10 ^ -21 mol) within a wide dynamic range (6 orders of magnitude), with sensitivity increased by 50 times compared to traditional designs.

2. Quality analyzer

• Triple quadrupole architectureQuality range of 5-2000 Da, scanning speed of 10000 amu/sec (FASST mode), supports synchronous monitoring of 16384 MRM channels, and the dwell time can be shortened to 1 ms, matching the fast separation requirements of UHPLC.

• Segmented collision poolUsing a hexapole collision reaction cell (CRC) to eliminate cross interference, with collision energy accuracy of ± 0.1 eV, ensuring fragment ion reproducibility (RSD<0.3%).

2、 Intelligent functions and analysis methods

1. Dynamic Multi Reaction Monitoring (dMRM)

• A single method can quantify thousands of compounds, automatically adjust residence time and period, solve the problem of co effluent interference, and is suitable for complex pesticide residue screening standards such as EU SANTE/11813/2017.

2. Triggered MRM (tMRM)

• Simultaneously triggering sub ion scanning in MRM quantification, combined with NIST spectral library to achieve compound confirmation, meets the compliance requirements of 33 prohibited pesticide residues in the document.

3. MassHunter Optimizer

• Automatically optimize MRM parameters (CE, DP voltage, etc.), develop methods for 100 compounds within 30 minutes, and increase efficiency by 90%.

3、 Application scenarios and performance validation

1. Pharmaceutical and Clinical Research

• The sensitivity of biomarker validation reaches 0.01 ng/mL (such as in plasma), supporting PK/PD research; The accuracy of DAR value for complete antibody analysis is ± 0.1 (optional BioPharma kit).

2. food safety

• Quantification of pesticide residues at 0.1 pg level (such as fipronil), according to GB 23200.113 standard, can complete high-throughput screening of 500+samples in a single day.

3. environmental monitoring

• The detection limit for 117 VOCs specified by EPA 8260B is as low as 0.25 pg, and the analysis of dioxins complies with EU 589/2014 regulations.

4、 Advantages of System Expansion and Maintenance

• modular designCan be upgraded to QTRAP system (MRM ³ mode), or integrated with FAIMS Pro ion mobility interface to enhance the selectivity of complex matrices.

• NeverVent TechnologyNo need to release vacuum to replace ion source or chromatography column, maintenance time<5 minutes, annual downtime reduced by 70%.

summary

The Agilent 6400 series, with its iFunnel technology, 10000 level MRM channels, and intelligent softwareTrace quantification (Ak level), analytical flux (1 ms residence)andLong term stability (RSD<1%)Upward establishment, especially suitable for laboratories with high regulatory requirements. Its low operation and maintenance costs (reducing energy consumption by 30%) and flexible scalability further enhance market competitiveness